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Purified anti-CD276 (B7-H3) Antibody

Pricing & Availability
Clone
A20079E (See other available formats)
Regulatory Status
RUO
Other Names
Cluster of differentiation 276 (CD276), B7 Homolog 3 (B7-H3), 4Ig-B7-H3, B7RP-2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A20079E_PURE_CD276_IHCP_070723
IHC staining of purified anti-CD276 (B7-H3) (clone A20079E) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured in an Olympus IX83 Inverted Microscope with a 20X objective. Scale bar: 50 µm
  • A. A20079E_PURE_CD276_IHCP_070723
    IHC staining of purified anti-CD276 (B7-H3) (clone A20079E) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured in an Olympus IX83 Inverted Microscope with a 20X objective. Scale bar: 50 µm
  • B. A20079E_PURE_CD276_FC_070723
    HaCat cells (positive control, filled histogram) and Jurkat cells (low expression control, open histogram) were stained with purified anti-CD276 (B7-H3) (clone A20079E ), or mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat No. 401402) followed by PE goat anti-mouse IgG (Cat. No. 405307).
  • C. A20079E_PURE_CD276_ICC_070723
    ICC staining of purified anti-CD276 (B7-H3) (clone A20079E ) on HaCaT cells. The cells were fixed and permeabilized with ice-cold MeOH, then blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with either 5 µg/mL of purified mouse IgG1, κ isotype control (Cat. No. 401402) (panel A) or 1.25 µg/mL of the primary antibody (panel B) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the images were captured with a 60X objective. Scale: 50 µm
  • D. A20079E_PURE_CD276_WB_070723.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-CD276 (B7-H3) (clone A20079E ) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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616951 25 µg £124
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616952 100 µg £308
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Description

CD276, or B7-H3, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human CD276 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain and a signature intracellular domain. There also exists an additional isoform known as 4Ig-B7-H3, containing a nearly exact tandem duplication of the IgV-IgC domain. Expression of CD276 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages, and is upregulated during the maturation of monocytes to DCs, or following interactions between DCs and regulatory T cells. CD276 inhibits T-cell responses, and plays a role in adaptive immunity by suppressing T cell activation. CD276 is also expressed in various cancers, where it works to suppress tumor antigen-specific immune responses. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human CD276
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
FC, ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A20079E does not cross-react with mouse CD276 in WB. 

RRID
AB_3068218 (BioLegend Cat. No. 616951)
AB_3068218 (BioLegend Cat. No. 616952)

Antigen Details

Structure
CD276 is a 534 amino acid protein with a predicted molecular weight of 58.2 kD.
Distribution

Dendritic cells, macrophages, natural killer cells, induced T cells. In the tonsil, it is expressed in epithelial cells.

Function
Co-stimulatory molecule that inhibits T-cell responses
Ligand/Receptor
CD276 binds to Trem-like transcript 2 (TLT-2).
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Inhibitory Molecules, Innate Immunity
Antigen References
  1. Nagashima O, et al. 2008. J Immunol. 181:4062-71.
  2. Prasad DVR, et al. 2004. J Immunol. 173:2500-6.
  3. Sun M, et al. 2002. J Immunol. 168:6294-7.
  4. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235-40.
  5. Ford JW, et al. 2009. Curr Opin Immunol. 21:38-46. 
  6. Leitner J, et al. 2009. Eur J Immunol. 2009. 39:1754-64.
Gene ID
80381 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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