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Purified anti-CD31 (PECAM-1) Antibody

Pricing & Availability
Clone
P2B1 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet endothelial cell adhesion molecule, PECA1, GPIIA', endoCAM, CD31 antigen, CD31/EndoCAM, adhesion molecule, PECAM-1, CD31/EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P2B1_Pure_CD31_PECAM1_Antibody_010516
IHC staining of clone P2B1 on frozen human kidney tissue. The primary antibody was incubated at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP Kit (Multi-Species) was used for detection according to the protocol provided.
  • P2B1_Pure_CD31_PECAM1_Antibody_010516
    IHC staining of clone P2B1 on frozen human kidney tissue. The primary antibody was incubated at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP Kit (Multi-Species) was used for detection according to the protocol provided.
  • P2B1_Pure_CD31_PECAM1_Antibody_2_010516
    Immunofluorescence of HUVEC cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) CD31 antibody (Clone P2B1). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/15, and (B-D) is 1/50. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml and (D) is 0.5 µg/ml.
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910005 100 µg £174
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Description

CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam, is a 130-140 kD type I transmembrane glycoprotein. It is expressed on monocytes, platelets, granulocytes, endothelial cells, and lymphocyte subsets. In inflammatory situations, CD31 has been reported to bind CD38 and is involved in wound healing, angiogenesis, and cellular migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P2B1 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry on frozen tissue sections. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is recommended. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/ml (1:250 - 1:500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent staining1.


Predicted Molecular Weight = 83 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Brooks PC, et al. 1995. J. Clin. Invest. 96(4):1815. (IF)
RRID
AB_2566676 (BioLegend Cat. No. 910005)

Antigen Details

Structure
130-140 kD type I transmembrane glycoprotein belonging to the Ig superfamily.
Distribution

Found on the surface of platelets, monocytes, neutrophils, and some types of T-cells. Makes up a large portion of endothelial cell intercellular junctions.

Function
Cell adhesion molecule which is required for leukocyte transendothelial migration under most inflammatory conditions. Induces susceptibility to atherosclerosis, member of the immunoglobulin superfamily and is involved in leukocyte migration, angiogenesis, and integrin activation.
Ligand/Receptor
CD38
Cell Type
Platelets, Monocytes, Neutrophils, T cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroinflammation, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tang DG, et al. 1993. J. Biol. Chem. 268(30):22883.
2. Wenzel K, et al. 1999. Hum. Mutat. 14(6):545.

Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 1    Revision Date: 07/20/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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