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Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) Antibody

Pricing & Availability
Clone
Poly22A02 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase erbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185erbB2, cluster of differentiation 340 (CD340), NGL, VSCN2, TKR1
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly22A02_PURE_CD340_Antibody_073123
Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated with 100 ng Neuregulin (+) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.125 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) (clone Poly22A02) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly22A02_PURE_CD340_Antibody_073123
    Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated with 100 ng Neuregulin (+) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.125 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) (clone Poly22A02) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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616251 50 µL £172
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Description

CD340, also commonly known as ErbB2 or HER2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of CD340 in breast cancers has been shown to confer Taxol resistance, and CD340 is a therapeutic target in a number of human cancers. CD340 activation is accompanied by autophosphorylation at multiple tyrosine residues including Tyr1196.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide of human CD340 (ErbB2/HER-2) phosphorylated at Tyr1196
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution range of this reagent is 1:500 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068237 (BioLegend Cat. No. 616251)

Antigen Details

Structure
CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution

Plasma membrane

Function
Proto-oncogene
Interaction
CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules, Phospho-Proteins
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org
Go To Top Version: 1    Revision Date: 07/31/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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