- Clone
- A22023C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
639251 | 25 µg | £107 | ||||
639252 | 100 µg | £264 |
CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confer Taxol resistance. CD340 autophosphorylation at Tyr1196 mediates Crk interaction that leads to Erk activation. In addition, CD340 phosphorylation has been related with polarity disruption, cell migration and metastasis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human CD340 phosphorylated at Tyr1196
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3662323 (BioLegend Cat. No. 639251)
AB_3662323 (BioLegend Cat. No. 639252)
Antigen Details
- Structure
- CD340 is a 1,255 amino acid protein with a predicted molecular weight of 185 kD. It is a glycosylated receptor-like transmembrane protein.
- Distribution
-
Cellular membrane and also present in internalized vesicles
- Function
- Receptor, proto-oncogene
- Interaction
-
Homodimers
Heterodimers with EGFR, ErbB3, ErbB4 PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL - Ligand/Receptor
- Epidermal growth factor family
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
- Molecular Family
- Adaptor Proteins, CD Molecules, Phospho-Proteins
- Antigen References
-
- Akiyama T, et al. 1986. Science. 232:1644-6.
- Bargmann CI, et al. 1986. Nature. 319:226-30.
- Pegram MD, et al. 1997. Oncogene. 15:537-47.
- Slamon DJ, et al. 1989. Science. 244:707-12.
- Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
- Gene ID
- 2064 View all products for this Gene ID
- UniProt
- View information about CD340 on UniProt.org
Related FAQs
Other Formats
View All CD340 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) | A22023C | WB |
Compare Data Across All Formats
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Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196)
Whole cell extracts (15 µg total protein) from MCF-7 cells u...
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