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Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) Antibody

Pricing & Availability
Clone
A22023C (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A22023C_PURE_CD340_WB_061124
Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated (+) with Neuregulin 1 (Cat. No. 765704) to induce CD340 phosphorylation were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) (clone A22023C) (left blot) or pan Purified anti-CD340 (ErbB2/HER-2) (right blot). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • A22023C_PURE_CD340_WB_061124
    Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated (+) with Neuregulin 1 (Cat. No. 765704) to induce CD340 phosphorylation were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) (clone A22023C) (left blot) or pan Purified anti-CD340 (ErbB2/HER-2) (right blot). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
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639251 25 µg £107
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639252 100 µg £264
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Description

CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confer Taxol resistance. CD340 autophosphorylation at Tyr1196 mediates Crk interaction that leads to Erk activation. In addition, CD340 phosphorylation has been related with polarity disruption, cell migration and metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human CD340 phosphorylated at Tyr1196
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662323 (BioLegend Cat. No. 639251)
AB_3662323 (BioLegend Cat. No. 639252)

Antigen Details

Structure
CD340 is a 1,255 amino acid protein with a predicted molecular weight of 185 kD. It is a glycosylated receptor-like transmembrane protein.
Distribution

Cellular membrane and also present in internalized vesicles

Function
Receptor, proto-oncogene
Interaction
Homodimers
Heterodimers with EGFR, ErbB3, ErbB4
PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL
Ligand/Receptor
Epidermal growth factor family
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
Molecular Family
Adaptor Proteins, CD Molecules, Phospho-Proteins
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org
Go To Top Version: 1    Revision Date: 06/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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