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Purified anti-CD36L1 (SCARB1, SR-BI) Antibody

Pricing & Availability
Clone
O91E1 (See other available formats)
Regulatory Status
RUO
Other Names
SRB1, SR-B1, SRBI, CLA-1, CLA1, HDLQTL6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Total cell lysates (15 µg total protein) from RPMI 8226, A549, THP-1, and U87-MG cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-CD36L1(SCARB1, SR-BI) Antibody, clone O91E1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted CD36L1 expression; predicted expression data was obtained from Human Protein Atlas.
  • Total cell lysates (15 µg total protein) from RPMI 8226, A549, THP-1, and U87-MG cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-CD36L1(SCARB1, SR-BI) Antibody, clone O91E1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted CD36L1 expression; predicted expression data was obtained from Human Protein Atlas.
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678702 100 µg £230
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Description

Scavenger receptor class B type I (SCARB1), also known as CD36L1, is an integral membrane protein found in numerous cell types and tissues including the liver and the adrenal glands. Its primary function is to facilitate the uptake of cholesteryl esters from high-density lipoproteins (HDL) due to its high affinity to HDL. This function serves to lower the risk of development of atherosclerosis. SCARB1 is also able to bind to other lipoproteins such as LDL, OxLDL, and VLDL. SCARB1 is also likely to be involved in pathogen recognition through its recognition of mycobacteria and other viral proteins and regulates vitamin E levels in the tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human SCARB1 protein produced in HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566049 (BioLegend Cat. No. 678702)

Antigen Details

Structure
Different isoforms with a predicted molecular weight of approximately 60 kD to 80 kD. Integral membrane glycoprotein.
Distribution

Macrophages, hepatocytes, dendritic cells, adrenocortical cells, adipocytes, and trophoblastic cells.

Function
Involved in the uptake of high density lipoproteins (HDL), phagocytosis of apoptotic cells, and pathogen recognition. Receptor target of the hepatitis C virus.
Interaction
HCV E1/E2 and PDZK1.
Ligand/Receptor
Phosphatidylserine, HDL, apoB-containing lipoproteins, LDL, and vitamin E.
Cell Type
Dendritic cells, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Acton S, et al. 1996. Science 5248:518.
2. Rigotti A, et al. 2003. Endocr. Rev. 24:357.
3. Valacchi G, et al. 2011. Ann. NY Acad. Sci. 1229:E1.
4. Scarselli E, et al. 2002. EMBO J. 21:5017.
5. Mardones P, et al. 2002. J. Nutr. 132:443.

Gene ID
949 View all products for this Gene ID
UniProt
View information about CD36L1 on UniProt.org
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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