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Purified anti-CD99 (Ewing's Sarcoma Marker) Antibody

Pricing & Availability
Clone
O13 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
CD99 antigen, E2 antigen, surface antigen MIC2, T-cell surface glycoprotein E2, antigen identified by monoclonal 12E7, Y homolog, antigen identified by monoclonal antibodies 12E7, F21, O13
Isotype
Mouse IgG1, κ
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Product Citations
publications
O13_PURE_CD99_Antibody_1_IHCP_061016
IHC staining of anti-CD99 antibody (clone O13) on formalin-fixed paraffin embedded human Ewing sarcoma tissue. Following antigen retrieval using BioLegend's Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody for 20 minutes at room temperature at 2.7 µg/ml. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection, followed by counterstaining with hematoxylin.
  • O13_PURE_CD99_Antibody_1_IHCP_061016
    IHC staining of anti-CD99 antibody (clone O13) on formalin-fixed paraffin embedded human Ewing sarcoma tissue. Following antigen retrieval using BioLegend's Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody for 20 minutes at room temperature at 2.7 µg/ml. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection, followed by counterstaining with hematoxylin.
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915604 100 µg £157
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Description

O13 detects a cell surface antigen expressed in 95% of Ewing's sarcomas (ES). The antigen is also found in some primative neuroectodermal tumors (PNET) and peripheral neuroepitheliomas (PN). Other small round cell tumors (SRCT) that are commonly included in the differential diagnosis of ES, such as neuroblastomas, lymphomas, and rhabdomyosarcomas, as well as other sarcomas, carcinomas, and neuroectodermal tumors are generally negative. In normal tissues, the antigen is strongly expressed in thymocytes and a subset of pancreatic islet cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against MeWo human melanoma cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for p30/32 MIC2, a 30-32 kD glycoprotein, also referred to as the HBA71 antigen.

Application References

(PubMed link indicates BioLegend citation)
  1. Fellinger EJ, et al. 1992. Am. J. Surg. Pathol. 16(8):746. PubMed
  2. Fellinger EJ, et al. 1991. Cancer Res. 51:336. PubMed
  3. Fellinger EJ, et al. 1991. Am. J. Surg. Pathol. 139(2):317. PubMed
  4. Dracopoli NC, et al. 1985. J. Hum. Genet. 37:199.
RRID
AB_2616966 (BioLegend Cat. No. 915604)

Antigen Details

Structure
CD99 is a 185 amino acid protein with a molecular mass of 19 kD.
Distribution

Tissue distribution: highly expressed in thymocytes, T cells, T cell leukemias and lymphomas.
Cellular distribution: plasma membrane and cytoskeleton.

Function
Involved in spontaneous rosette formation with erythrocytes. May be involved in other T-cell and hematopoietic cell adhesion pathways. Has been reported to activate a caspase-independent death pathway in T cells under some conditions.
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
4267 View all products for this Gene ID
UniProt
View information about CD99 on UniProt.org
Go To Top Version: 2    Revision Date: 06/17/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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