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Purified anti-CDA Antibody

Pricing & Availability
Clone
W20116C (See other available formats)
Regulatory Status
RUO
Other Names
CDA, Cytidine Deaminase, Cytidine Aminohydrolase, Cytosine Nucleoside Deaminase, Small Cytidine Deaminase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W20116C_PURE_CDA_WB_102424
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CDA (clone W20116C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W20116C_PURE_CDA_WB_102424
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CDA (clone W20116C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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660551 25 µg £100
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660552 100 µg £278
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Description

CDA, Cytidine deaminase, is an enzyme that plays a critical role in nucleic acid metabolism by catalyzing the deamination of cytidine to uridine. This reaction involves the removal of an amine group from cytidine, converting it into uridine and releasing ammonia as a byproduct. The importance of CDA lies in its involvement in several essential biological processes, including RNA editing, DNA repair, and pyrimidine metabolism.

CDA is integral to the catabolic pathway of pyrimidine nucleotides, particularly the salvage pathway, which recycles nucleotides for DNA and RNA synthesis. Cytidine deaminase breaks down cytidine and deoxycytidine into uridine and deoxyuridine, respectively. These products can then be further processed to form bases and nucleosides for the maintenance of nucleotide pools. By regulating pyrimidine homeostasis, CDA ensures that cells have a balanced supply of nucleotides, which is essential for DNA replication, transcription, and overall cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human CDA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It is not recommended to use this product for Immunohistochemistry (IHC), immunocytochemistry (ICC), or intracellular flow cytometry (ICFC).

This antibody is not recommended for use in Western blotting (WB) with mouse samples due to the presence of an unidentified band around 10 kDa.

Antigen Details

Structure
Homotetramer
Distribution

Expressed in lymphoid organs, liver, and esophagus. Located in the cytoplasm

Function
Scavenges cytidine and 2'-deoxycytidine for UMP synthesis
Biology Area
Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Schröder J K, et al. 1998. Br J Haematol. 103:1096-103.
  2. Bardenheuer W, et al. 2005. Leukemia. 19:2281-8.
  3. Mameri H, et al. 2017. Clin Cancer Res. 23:2116-2126.
Gene ID
978 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 1    Revision Date: 10/24/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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