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Purified anti-CDK1 Antibody

Pricing & Availability
Clone
A20004C (See other available formats)
Regulatory Status
RUO
Other Names
Cell division cycle protein 2 (CDC2), G1 to S and G2 to M, cyclin dependent kinase 1 (CDK1), cdc28A, p34 kinase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20004C_PURE_CDK1_Antibody_012722
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CDK1 (clone A20004C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A20004C_PURE_CDK1_Antibody_012722
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CDK1 (clone A20004C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A20004C_PURE_CDK1_Antibody_2_012722
    ICC staining of purified anti- CDK1 (clone A20004C) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 10 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. The image was captured with a 60X objective.
  • A20004C_PURE_CDK1_Antibody_3_021422
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG2b, κ isotype control (Cat. No. 400302) or purified anti-CDK1 (clone A20004C). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a mouse control antibody against a separate epitope of CDC2. Lane M: Molecular weight marker
  • A20004C_PURE_CDK1_Antibody_4_121422
    IHC staining of purified anti-CDK1 (clone A20004C) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5.0 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
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602351 25 µg £81
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602352 100 µg £201
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Description

Cyclin-dependent kinase 1 (CDK1) is a serine/threonine kinase that is ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that universally induces entry into mitosis in eukaryotes. CDK1 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. In addition to regulation by protein-protein interactions, CDK1 is controlled by phosphorylation, particularly at conserved tyrosine residues. In humans, CDK1 is encoded by the gene CDC2 (cell division control protein homolog 2).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human CDK1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product was tested and did not produce a detectable signal for mouse cross-reactivity in WB.

This product was tested and did not produce a detectable signal for Immunoprecipitation.

This product was tested and did not produce a detectable signal in IHC in human skin tissue.

RRID
AB_2922631 (BioLegend Cat. No. 602351)
AB_2922631 (BioLegend Cat. No. 602352)

Antigen Details

Structure
CDK1 is a 297 amino acid protein with an estimated molecular weight of 34.1 kD.
Distribution

CDK1 is expressed throughout the cytoplasm and nucleus.

Function
CDK1 is a serine/threonine kinase.
Interaction
CDK1 phosphorylates a myriad of targets including transcription factors, other kinases, enzymes, and structural and nuclear proteins associated with mitosis. Additionally, CDK1 is regulated by other kinases, most notably Wee1, which inhibits CDK1 activity.
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Malumbres M. 2014. Genome Biol. 15:122.
  2. Draetta G, et al. 1988. Nature. 336:738-44.
Gene ID
983 View all products for this Gene ID
UniProt
View information about CDK1 on UniProt.org

Other Formats

View All CDK1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-CDK1 A20004C WB,ICC,IP,IHC-P
Go To Top Version: 2    Revision Date: 12/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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