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Purified anti-Chil3 Antibody

Pricing & Availability
Clone
A17046B (See other available formats)
Regulatory Status
RUO
Other Names
Chitinase-like protein 3, Chil3, Beta-N-acetylhexosaminidase Ym1, Ym1, ECF-L, Eosinophil chemotactic cytokine
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17046B_PURE_Chil3_Antibody_1_072018
Western blot of purified anti-Chil3 antibody (clone W17046B). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse spleen lysate; Lane 3: 20 µg of human spleen lysate; lane 4: 10 ng of recombinant mouse Chil3. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Cytochrome C (Cat. No. 612504) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • A17046B_PURE_Chil3_Antibody_1_072018
    Western blot of purified anti-Chil3 antibody (clone W17046B). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse spleen lysate; Lane 3: 20 µg of human spleen lysate; lane 4: 10 ng of recombinant mouse Chil3. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Cytochrome C (Cat. No. 612504) was used as loading control. Enhanced chemiluminescence was used as the detection system.
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853801 25 µg £73
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853802 100 µg £189
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Description

Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin. Chitinase-like protein 3 (Chil3) hydrolyzes terminal non-reducing N-acetyl-D-hexosamine residues into N-acetyl-beta-D-hexosaminides. This protein is particular enriched in the spleen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse Chil3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2750120 (BioLegend Cat. No. 853801)
AB_2750120 (BioLegend Cat. No. 853802)

Antigen Details

Structure
Chil3 is a 398 amino acid protein with a molecular weight mass of ~45 kD.
Distribution

Tissue distribution: Spleen, macrophages, and bone marrow-derived neutrophils.
Cellular distribution: Nucleus, endoplasmic reticulum, and secreted.

Function
Chil3 plays a role in hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Interaction
Chil3 binds to chitin, lectin, and heparin.
Cell Type
Macrophages, Neutrophils
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Enzymes and Regulators
Antigen References
  1. Mesci P, et al. 2015. Brain. 138:53-68
  2. Wu F, et al. 2016. Parasitol Res. 115:3737
  3. Al-Rubaie A, et al. 2018. Respir Res. 19:114
Gene ID
12655 View all products for this Gene ID
UniProt
View information about Chil3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/23/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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