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Purified anti-CHOP Antibody

Pricing & Availability
Clone
9C8/CHOP (See other available formats)
Regulatory Status
RUO
Other Names
DDIT3, GADD153
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_9C8slashCHOP_PURE_CHOP_Antibody_WB_100721.png
Whole cell extracts (15 µg protein) from HeLa and HEK-293, and RAW264.7 cells untreated (-) (negative control) or treated with 2.0 µg/mL tunicamycin for 8 hours (+) (positive control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CHOP antibody (clone 9C8/CHOP) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
  • 1_9C8slashCHOP_PURE_CHOP_Antibody_WB_100721.png
    Whole cell extracts (15 µg protein) from HeLa and HEK-293, and RAW264.7 cells untreated (-) (negative control) or treated with 2.0 µg/mL tunicamycin for 8 hours (+) (positive control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CHOP antibody (clone 9C8/CHOP) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
  • 2_9C8slashCHOP_PURE_CHOP_Antibody_ICC_100721.png
    ICC staining of purified anti-CHOP antibody (clone 9C8/CHOP) on untreated (panel A) and tunicamycin treated (2.0 µg/mL, 8 hours) (panel B) HeLa cells cells. The cells were fixed with 4% PFA and permeabilized with Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • 3_9C8slashCHOP_PURE_CHOP_Antibody_ICFC_100721.png
    HeLa cells treated with 2.0 µg/mL tunicamycin for 8 hours (filled histogram) and untreated HeLa cells (open histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with purified anti-CHOP antibody (clone 9C8/CHOP) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307).
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948701 25 µg £81
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948702 100 µg £201
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Description

When unfolded proteins accumulate in the ER lumen, multiple unfolded protein response (UPR) pathways become activated, including PERK, ATF6, and IRE1α, all of which transcriptionally reprogram the flux of proteins through the ER. When these quality control mechanisms fail to restore ER homeostasis, sustained stress results in the induction of CHOP, a pro-apoptotic transcription factor that negatively regulates expression of BCL2 and increases expression of BIM and TNFS10RB. One mechanism cancer cells exploit to adapt to harsh conditions of the tumor microenvironment is through upregulation of UPR pathways. Thus, understanding the molecular mechanisms that govern the switch from adaptation to apoptosis is of great therapeutic interest.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human CHOP protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.5 - 1.0 µg/mL. For immunocytochemistry, a concentration 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed to detect CHOP in human small intestine.

For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.

RRID
AB_2904462 (BioLegend Cat. No. 948701)
AB_2904462 (BioLegend Cat. No. 948702)

Antigen Details

Structure
CHOP is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution

Expressed in cells and tissue undergoing ER stress/Nucleus

Function
Transcription Factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Misfolding and Aggregation, Transcription Factors
Antigen References

1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495
2. Oyadomari, et al. 2004. Cell Death Differ. 11:381.
3. Puthalakath H, et al. 2007. Cell. 129: 1337.

Gene ID
1649 View all products for this Gene ID
UniProt
View information about CHOP on UniProt.org
Go To Top Version: 1    Revision Date: 10/07/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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