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Purified anti-CKMT2 Antibody

Pricing & Availability
Clone
3F4-G5-H5 (See other available formats)
Regulatory Status
RUO
Other Names
Creatine Kinase, Mitochondrial 2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3F4-G5-H5_PURE_CKMT2_Antibody_1_080219
Western blot of purified anti-CKMT2 antibody (clone 3F4-G5-H5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F4-G5-H5_PURE_CKMT2_Antibody_1_080219
    Western blot of purified anti-CKMT2 antibody (clone 3F4-G5-H5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F4-G5-H5_PURE_CKMT2_Antibody_2_080219
    IHC staining of purified anti-CKMT2 antibody (clone 3F4-G5-H5) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 3F4-G5-H5_PURE_CKMT2_Antibody_3_070620
    ICC staining of purified anti-CKMT2 (clone 3F4-G5-H5) on SH-SY5Y cell. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
868701 25 µg £73
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868702 100 µg £189
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Description

CKMT2 is an isoform of the mitochondrial specific creatine kinase subunit that is mainly expressed in skeletal and heart muscle cells. CKMT2 exists in two different oligomeric forms: dimers and octamers. Recent studies have shown that the homooctameric form of CKMT2 is the predominant form in vivo and is critical to its function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration of 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814623 (BioLegend Cat. No. 868701)
AB_2814623 (BioLegend Cat. No. 868702)

Antigen Details

Structure
CKMT2 is a 419 amino acid protein with predicted and observed molecular mass of ~47 kD.
Distribution

Tissue Distribution: Mainly expressed in heart and skeletal muscle. Also expressed in brain.
Cellular Distribution: Mitochondrion and cytosol

Function
CKMT2 plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain.
Interaction
CKMT2 exists as octamer composed of four homodimers.
Biology Area
Mitochondrial Function, Neurodegeneration, Neuroscience
Molecular Family
Mitochondrial Markers
Antigen References
  1. Zervou S, et al. 2017. PLoS One. 12(8):e0182994
  2. Forsey KE, et al. 2013. Mol Reprod Dev. 80(3):185
  3. Miceli MV, et al. 2005. Exp Cell Res. 302 (2):270
Gene ID
1160 View all products for this Gene ID 76722 View all products for this Gene ID 688698 View all products for this Gene ID
UniProt
View information about CKMT2 on UniProt.org
Go To Top Version: 2    Revision Date: 07/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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