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Purified anti-Claudin-6 Antibody

Pricing & Availability
Clone
W22275B (See other available formats)
Regulatory Status
RUO
Other Names
Claudin-6, Claudin 6, CLDN6, Skullin
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W22275B_PURE_Claudin6_Antibody_1_100824
Whole cell extracts (15 μg total protein) from the indicated cell lines were resolved on a 12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-Claudin-6 (clone W22275B). Proteins were visualized by chemiluminescence detection using an HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22275B_PURE_Claudin6_Antibody_1_100824
    Whole cell extracts (15 μg total protein) from the indicated cell lines were resolved on a 12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-Claudin-6 (clone W22275B). Proteins were visualized by chemiluminescence detection using an HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22275B_PURE_Claudin6_Antibody_2_100824
    IHC staining of Claudin-6 was performed on formalin-fixed paraffin embedded human ovarian cancer tissue following antigen retrieval using Citrate buffer (Cat. No. 420902). The tissue sections were incubated with Purified anti-Claudin-6 (clone W22275B) (panel B) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416) or secondary antibody only (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A and overlay in panel C). Scale Bar: 50 µm
  • W22275B_PURE_Claudin6_Antibody_3_100824
    HeLa cells (negative cell line, panel A) and OVCAR-3 cells (positive cell line, panels B and C) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100 and then blocked with 5% FBS. Cells were stained with Purified anti-Claudin-6 (clone W22275B) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801)(overlay in panels A and C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 μm
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650051 25 µg £124
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650052 100 µg £305
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Description

Claudin-6 is a member of the claudin family of proteins, which consists of at least 24 members. Claudin proteins are integral membrane proteins characterized by 4 transmembrane regions and 2 extracellular loops that form tight junctions. Abnormal expression can disrupt tight junction integrity, playing an important role in the occurrence and development of cancers. Claudin-6 in particular, has been considered a therapeutic target because it is commonly expressed in tumor tissue but not in healthy adult tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Claudin-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 10 - 1 ug/mL is suggested. For immunocytochemistry, a concentration range of 10 - 1 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using PFA-Triton X-100 and Methanol. We do not recommend PFA-Methanol fixation/permeabilization.

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA buffer.

Antigen Details

Structure
Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa.
Distribution

Cellular membrane

Function
Tight junctions and cell adhesion
Antigen References
  1. Reinhard K, et al. 2020. Science. 367(6476):446-453.
  2. Huan D, et al. 2021. Mol Med Rep. 24(3):677.
  3. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56.
  4. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53.
Gene ID
9074 View all products for this Gene ID
UniProt
View information about Claudin-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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