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Purified anti-Connexin 43, 360-382 Antibody

Pricing & Availability
Clone
P2C4 (See other available formats)
Regulatory Status
RUO
Other Names
GJA1, Cx43, Gap Junction Protein Alpha 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P2C4_PURE_Connexin_Antibody_1_WB_061417_resized
Western blot of purified anti-Connexin 43, 360-382 antibody (clone P2C4), and isotype-matched IgG2a, κ control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate. Blots were incubated with 2 µg/ml of each primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • P2C4_PURE_Connexin_Antibody_1_WB_061417_resized
    Western blot of purified anti-Connexin 43, 360-382 antibody (clone P2C4), and isotype-matched IgG2a, κ control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate. Blots were incubated with 2 µg/ml of each primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • P2C4_PURE_Connexin_Antibody_2_IHC_012122
    IHC staining of purified anti-Connexin 43, 360-382 antibody (clone P2C4) on formalin-fixed, paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
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849601 25 µg £81
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849602 100 µg £201
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Description

Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to amino acids 360-382 of Connexin 43.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody binds to the C-terminus of Connexin 43 and shows preference for non-phosphorylated Connexin 43 by western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Chu G, et al. 2002. Cardiovasc. Res. 54(1):105. (WB)
  2. Wei CJ, et al. 2005. J. Biol. Chem. 280(20):19925. (WB)
RRID
AB_2687296 (BioLegend Cat. No. 849601)
AB_2687296 (BioLegend Cat. No. 849602)

Antigen Details

Structure
Connexin 43 (GJA1) is a 382 amino acid protein with a molecular mass of 43 kD.
Distribution

Tissue distribution: Expressed in most tissues, enriched in brain, endocrine, muscle, bone marrow, immune System, ovaries, testes, and Skin.

Cellular distribution: Plasma membrane, cytosol, mitochondria, golgi apparatus, cytoskeleton and endoplasmic reticulum membrane.

Function
Connexin 43 plays a role in cell-to-cell communication by forming channels, or gap junctions between cells. It allows for the transport of nutrients, charged particles (ions), and other small molecules that carry necessary communication signals between cells.
Molecular Family
Gap Junction Proteins
Antigen References

1. Grek CL, et al. 2016. Cancer Lett. 374(1):117.
2. Lilly E, et al. 2016. Semin. Cell Dev. Biol. Feb; 50:4
3. Michela P, et al. 2015. Eur. J. Pharmacol. 768:71-6.

Gene ID
2697 View all products for this Gene ID
UniProt
View information about Connexin 43 360-382 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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