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Purified anti-CREB1 Antibody

Pricing & Availability
Clone
A18233D (See other available formats)
Regulatory Status
RUO
Other Names
CREB, CREB-1, cAMP responsive element binding protein 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A18233D_PURE_CREB1_Antibody_1_090921.png
Western blot of anti-CREB1 antibody (clone A18233D). Total cell lysates (15 µg total protein) from MOLT-4 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), HEK293 (Lane 4), and NIH/3T3 (Lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-CREB1 antibody overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25,000 dilution (lower). Lane M: Molecular weight ladder.
  • A18233D_PURE_CREB1_Antibody_1_090921.png
    Western blot of anti-CREB1 antibody (clone A18233D). Total cell lysates (15 µg total protein) from MOLT-4 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), HEK293 (Lane 4), and NIH/3T3 (Lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-CREB1 antibody overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25,000 dilution (lower). Lane M: Molecular weight ladder.
  • A18233D_PURE_CREB1_Antibody_2_090921.png
    IHC staining of anti-CREB1 antibody (clone A18233D) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 1.0 µg/mL of purified anti-CREB1 antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
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948301 25 µg £81
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948302 100 µg £201
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Description

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor binding to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human CREB1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes endogenous CREB1 proteins at 43 kD in both human and mouse cell lysates.

RRID
AB_2894537 (BioLegend Cat. No. 948301)
AB_2894537 (BioLegend Cat. No. 948302)

Antigen Details

Structure
CREB1 contains 327 amino acids at a predicted molecular weight at 35 kD.
Distribution

Ubiquitously expressed /Nucleus

Function
Under physiological conditions, CREB1 protein maintain cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving as a neuroprotective role. Under pathological conditions, increased level of CREB1 associates with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction
CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type
B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Andre S, et al. 2020. Cell Mol Life Sci. 77:4049-4067
  2. Pei-Hsuan L, et al. 2020. Diagnostics. 10:295
  3. Kensuke S, et al. 2011. J Neurochem. 116:1-9
  4. Andy YW, et al. 2010. J Immunol. 185:6413-6419
  5. TA, et al. 2002. EMBO J. 21:53-63 
Gene ID
1385 View all products for this Gene ID
UniProt
View information about CREB1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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