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Purified anti-CRISPR (Cas9) Antibody

Pricing & Availability
Clone
6G12-H11 (See other available formats)
Regulatory Status
RUO
Other Names
CRISPR associated protein 9, SpyCas9
Isotype
Mouse IgG1, κ
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Product Citations
publications
6G12-H11_PURE_CRISPR_Antibody_070717
Total cell lysates (15µg protein) from 293T and 293T transfected with Cas9 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1µg/ml) purified anti-CRISPR (Cas9) antibody (clone 6G12-H11,upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
  • 6G12-H11_PURE_CRISPR_Antibody_070717
    Total cell lysates (15µg protein) from 293T and 293T transfected with Cas9 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1µg/ml) purified anti-CRISPR (Cas9) antibody (clone 6G12-H11,upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
Cat # Size Price Quantity Check Availability Save
698301 25 µg £70
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698302 100 µg £174
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Description

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 interrogates and cleaves foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
S. Pyogenes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C-terminal recombinant fragment of S. pyogenes Cas9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml (1:1000 - 1:10,000). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2715788 (BioLegend Cat. No. 698301)
AB_2715788 (BioLegend Cat. No. 698302)

Antigen Details

Structure
The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound. The predicted Mw is 158kDa.
Distribution

S. Pyogenes

Function
Part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
Interaction
Binds crRNA and tracrRNA
Antigen References

1. Mali P, et al. 2013. Science 339:823.

Gene ID
901176 View all products for this Gene ID
UniProt
View information about CRISPR on UniProt.org
Go To Top Version: 0    Revision Date: 07/07/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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