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Purified anti-CRK Antibody

Pricing & Availability
Clone
W20038C (See other available formats)
Regulatory Status
RUO
Other Names
CRK, CRKII, p38, V-CRK Avian Aarcoma Virus CT10 Oncogene Homolog, CRK proto-oncogene adaptor protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W20038C_PURE_CRK_Antibody_WB_112222
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-CRK (clone W20038C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • A_W20038C_PURE_CRK_Antibody_WB_112222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-CRK (clone W20038C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • B_W20038C_PURE_CRK_Antibody_IHC_112222
    IHC staining of purified anti-CRK (clone W20038C) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Tris-buffered saline with Tween-20 (Cat. No. 925501), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_W20038C_PURE_CRK_Antibody_ICC_112222
    HepG2 cells were fixed with ice-cold methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL of either purified rat IgG2a, κ isotype control (Cat. No. 400502) (panel A) or purified anti-CRK (clone W20038C) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar: 50 µm
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609351 25 µg £107
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609352 100 µg £238
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Description

Adapter protein CRK, also known as CRK proto-oncogene adaptor protein or V-CRK avian sarcoma virus CT10 oncogene homolog, has two isoforms: CRKI (28 kD) and CRKII (40 kD). Closely associated is CRKL, or CRK-like protein (36 kD). CRKII and CRKL possess a C-terminal SH3 domain and a regulatory tyrosine phosphorylation site that are lacking in CRKI. CRK’s SH2 and SH3 domains serve as positive and negative regulators of transformation, respectively. CRK is vital to cell motility via activation of Rac1 by CRKII and CRKL and cell branching and adhesion via activation of Rap1 by CRKI. It is also vital to cell proliferation, apoptosis, formation and turnover of focal adhesions, PDGF-induced actin cytoskeleton rearrangement, cell migration, and transcription. Overexpressed in several cancers, CRK has been implicated in tumor proliferation, metastasis, and resistance to chemotherapy. This, in addition to its roles in natural killer cell activation and differentiation, lymphocyte regulation, B cell signaling, and T cell migration, makes CRK a valuable therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human CRK
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When testing by ICC, methanol rather than PFA fixation is recommended.

Clone W20038C is not recommended for immunoprecipitation.

RRID
AB_2928004 (BioLegend Cat. No. 609351)
AB_2928004 (BioLegend Cat. No. 609352)

Antigen Details

Structure
CRK is a 304 amino acid protein with a predicted molecular weight of 34 kD
Distribution

Ubiquitously expressed/ Cytoplasm, Membrane

Function
Cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1; proto-oncogene
Interaction
Rac1, Rap1, p130Cas, paxillin, Dock180, C3G/GRF2, ZAP70, Gab3, Cbl-b, HPK1, PI3K, PDGFRA, PDGFRB, IRS4, MAPK8, MAP4KI
Biology Area
Cell Biology
Molecular Family
Adaptor Proteins, Adhesion Molecules, Scaffold Proteins
Antigen References
  1. Antoku S and Mayer BJ. 2009. J Cell Sci. 122:4228-38.
  2. Liu, D. 2014. Immunol Cell Biol. 92:80-9.
  3. Park T. 2021. Cells. 10:739.
Gene ID
1398 View all products for this Gene ID
UniProt
View information about CRK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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