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Purified anti-CX3CR1 Antibody (Previously Covance catalog# MMS-5067)

Pricing & Availability
Clone
8E10.D9 (See other available formats)
Regulatory Status
RUO
Other Names
CX3C chemokine receptor 1, fractalkine receptor, beta chemokine receptor-like 1, chemokine (C-C) receptor-like 1, GRP13, V28
Previously
Covance Catalog# MMS-5067
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
8E10D9_Purified_CX3CR1_Antibody_IHC_030515
IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone 8E10.D9 at 20 μg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
  • 8E10D9_Purified_CX3CR1_Antibody_IHC_030515
    IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone 8E10.D9 at 20 μg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
Cat # Size Price Quantity Check Availability Save
824002 25 µL £77
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824001 100 µL £189
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Description

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, some malignant epithelial cells, astrocytes, and microglial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediates cell adhesion and migration. CX3CL1 has been reported to play an important role in neuroinflammation and neurodegeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 207-222 of the human CX3CR1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 10.0 - 20.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

Product Citations
  1. Pal B, et al. 2021. EMBO J. 40:e107333. PubMed
  2. Ahn JH, et al. 2019. Int J Mol Med. 44:939. PubMed
  3. Taylor JG, et al. 2022. Haematologica. :. PubMed
RRID
AB_2734592 (BioLegend Cat. No. 824002)
AB_2734592 (BioLegend Cat. No. 824001)

Antigen Details

Structure
Expected MW: 50 kD
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Gene ID
1524 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org
Go To Top Version: 7    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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