- Clone
- DCS-22 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- G1/S-specific cyclin-D3, CCND3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_DCS-22_PURE_CyclinD3_Antibody_WB_110117 1_DCS-22_PURE_CyclinD3_Antibody_WB_110117](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DCS-22_PURE_CyclinD3_Antibody_WB_110117.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Total lysates (15µg protein) from HeLa and HeLa Cyclin D3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti- Cyclin D3 antibody, clone DCS-22 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder. -
Total cell lysates (15 µg total protein) from HEPG2 (low expressing control), HeLa, A549 and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL (1:500 dilution) of Purified anti-Cyclin D3 Antibody, clone DCS-22, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
Protein (15 µg) harvested from Jurkat, K562, Daudi and NIH3T3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal Cyclin D3 (clone DCS-22) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody and chemiluminescence detection. -
PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and purified anti-Cyclin D3 (clone DCS-22) antibody (right) or purified mouse IgG1, κ isotype control (left). Followed by anti-mouse IgG PE. -
PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and purified anti-Cyclin D3 (clone DCS-22) antibody (left) or purified mouse IgG1, κ isotype control (right). Followed by anti-mouse IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
684902 | 100 µg | £182 |
D-type cyclins, including Cyclin D1, D2, and D3, are regulators of the Cyclin-dependent kinases 4 and 6 (Cdk4 and Cdk6) and mediate the growth factor-induced progression through the G1 phase in the cell cycle. Cyclins D1 and D3 are involved in cell-cycle progression in CDK-dependent manner and CDK-independent activation of transcription as well as metabolic control. CDKs promote cell cycle transitions in mammalian cells by phosphorylating key substrates cyclins. The active complex of cyclin D/CDK4 targets the retinoblastoma protein, which triggers the release of E2F transcription factors and activates G1/S-phase gene expression.
Abnormal expression of cyclins is involved in disease progression. Cyclin D3 is widely expressed in many tumor cells. Cyclin D3 gene is amplified in bladder carcinoma in situ. Genomic changes disrupting the expression of Cyclin D3 are associated with aberrant growth of several human B-lymphoid malignancies. Targeting Cyclin D3 by miR-138 could induce cell cycle arrest in hepatocellular carcinoma. Cyclin D3 was upregulated in breast cancer and could serve as predictor of breast cancer prognosis. Cyclin D3 can also be targeted in autoimmune diabetes and exerts a protective role on β cells by promoting their survival and fitness without affecting proliferation.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length Cyclin D3 of human origin.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC, KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2572190 (BioLegend Cat. No. 684902)
Antigen Details
- Structure
- Major isoform has 291 amino acids and predicted molecular weight of 33 kD.
- Distribution
-
Nucleus, cytoplasm and cell membrane.
- Function
- Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.
- Interaction
- CDK14, CDK4, CDK6, ATF5, EIF3K, CDKN1A, ICP0, POLR3GL, and TFIP11.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Antigen References
-
1. Liu W, et al. 2004. Biochem. Biophys. Res. Commun. 321:954.
2. Sherr CJ. 1993. Cell 73:1059.
3. Saavedra-Ávila NA. 2014. Proc. Natl. Acad. Sci. USA 111:E3405.
4. Lukas J, et al. 1996. Mol. Cell Biol. 16:6917.
5. Wang W, et al. 2012. Carcinogenesis. 33:1113.
6. Cato MH, et al. 2011. Mol. Cell Biol. 31:127.
7. Zhang Q, et al. 2014. Mol. Cell Endocrinol. 382:583.
8. Chi Y, et al. 2015. Cancer Cell Int. 15:89. - Gene ID
- 896 View all products for this Gene ID
- UniProt
- View information about Cyclin D3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Cyclin D3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cyclin D3 | DCS-22 | WB,ICFC,KO/KD-WB |
PE anti-Cyclin D3 | DCS-22 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Cyclin D3
Protein (15 µg) harvested from Jurkat, K562, Daudi and NIH3T... Total lysates (15µg protein) from HeLa and HeLa Cyclin D3 CR... Total cell lysates (15 µg total protein) from HEPG2 (low exp... PHA-stimulated (3 days) human peripheral blood mononuclear c... PHA-stimulated (3 days) human peripheral blood mononuclear c... -
PE anti-Cyclin D3
PHA-stimulated (3 days) human peripheral blood mononuclear c...
Follow Us