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Purified anti-Cyclin E1 Antibody

Pricing & Availability
Clone
W20169G (See other available formats)
Regulatory Status
RUO
Other Names
G1/S-specific cyclin-E1 (CCNE1), CCNE, pcyclin E1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W20169G_PURE_Cyclin-E1_IHC-P_040424
IHC staining of Purified anti-Cyclin E1 (clone W20169G) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-Cyclin E1 (clone W20169G) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • A. W20169G_PURE_Cyclin-E1_IHC-P_040424
    IHC staining of Purified anti-Cyclin E1 (clone W20169G) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-Cyclin E1 (clone W20169G) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • B. W20169G_PURE_Cyclin-E1_ICC_040424
    HeLa cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 µg/mL Purified rat IgG2a, κ isotype control (panel A) or 10 µg/mL of Purified anti-Cyclin E1 (clone W20169G) (panel B), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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634051 25 µg £113
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634052 100 µg £284
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Description

Cyclin E1 is a crucial regulator of cell cycle progression. Cyclins are proteins that play a pivotal role in the control of cell division by interacting with cyclin-dependent kinases (CDKs). Cyclin E1 forms a complex with CDK2 to phosphorylate target proteins and facilitate the initiation of DNA synthesis. Cyclin E1 specifically governs the G1-to-S phase transition, accumulating during G1 and then being degraded as the cell proceeds through S phase. This regulatory function ensures proper cell cycle progression and is essential for maintaining genomic stability. Aberrations in Cyclin E1 expression or activity have been associated with various cancers, emphasizing its significance in normal cellular processes and highlighting its potential as a therapeutic target in the context of cancer treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Cyclin E1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, the suggested use of this reagent is 1.25 - 10.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or Sodium Citrate (Cat. No. 928502).

For immunocytochemistry (ICC), we recommend fixation/permeabilization with either 100% ice-cold methanol, or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol.

This antibody (clone W20169G) is not suitable for Western blot (WB) or intracellular flow cytometry (ICFC).

RRID
AB_3106160 (BioLegend Cat. No. 634051)
AB_3106160 (BioLegend Cat. No. 634052)

Antigen Details

Structure
Cyclin E1 is a 410 amino acid protein with a predicted molecular weight of 47.1 kD.
Distribution

Nucleus

Function
Cell cycle regulation
Interaction
CDK2, UHRF2
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References
  1. Fagundes R and Teixeira LK. 2021. Front Cell Dev Biol. 9:774845.
  2. Lew DJ, et al. 1991. Cell. 66:1197-206. 
  3. Zeng J, et al. 2023. Cell. 186:528-542. 
  4. Chu C, et al. 2021. Trends Cell Biol. 31:732-746.
Gene ID
898 View all products for this Gene ID
UniProt
View information about Cyclin E1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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