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Purified anti-Cyclophilin D Antibody

Pricing & Availability
Clone
W17239A (See other available formats)
Regulatory Status
RUO
Other Names
Peptidylprolyl Isomerase F, Peptidyl-Prolyl Cis-Trans Isomerase F, Mitochondrial, Mitochondrial Cyclophilin, Cyclophilin F, Rotamase F, PPIase F, CyP-M, CYP3, CypD, Cyclophilin 3, Cyp-D, CyP-D, PPIF
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W17239A_PURE_Cyclophilin-D_Antibody_1_091119_updated
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Cyclophilin D Antibody, clone W17239A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending PPIF expression (Source: Human Protein Atals).
  • W17239A_PURE_Cyclophilin-D_Antibody_1_091119_updated
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Cyclophilin D Antibody, clone W17239A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending PPIF expression (Source: Human Protein Atals).
  • W17239A_PURE_Cyclophilin-D_Antibody_2_090919
    A-431 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502 panel A) or Purified anti-Cyclophilin D Antibody, clone W17239A (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-rat IgG Antibody (Cat. No. 405418) at 2.0 µg/mL and Alexa Fluor® 647 anti-Cytochrome c Antibody (Cat. No. 612310) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
670001 25 µg £70
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670002 100 µg £174
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Description

Cyclophilin D is one of the three components of the permeability transition pore (PTP) localized in the mitochondrial matrix. The opening of the PTP is a key event in the initiation of cell death which leads to mitochondrial swelling, outer membrane rupture, and the release of apoptotic regulators such as cytochrome C. Cyclophilin D promotes mitochondrial permeability transition (mPT) in response to increased Ca2+ level. Cyclophilin D deficient mice have shown desensitization to mTP and require twice as much Ca2+ intake for the opening of the PTP compared to wild-type mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PPIF protein (30-207 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes human cyclophilin D

This clone failed IP testing

Product Citations
  1. Kim S, et al. 2022. Cell Stress Chaperones. 27:573. PubMed
RRID
AB_2814520 (BioLegend Cat. No. 670001)
AB_2814520 (BioLegend Cat. No. 670002)

Antigen Details

Structure
Cyclophilin D is a 207 amino acid protein with a predicted molecular weight of 22 kD
Distribution

Mitochondrial matrix/Ubiquitously expressed

Function
Apoptotic regulator
Biology Area
Cell Death, Mitochondrial Function
Molecular Family
Mitochondrial Markers
Antigen References
  1. Baines C, et al. 2005. Nature. 434:658
  2. Schinzel A, et al. 2005. PNAS. 102:12005
  3. Basso E, et al. 2005. J BioL Chem. 280:18558
Gene ID
10105 View all products for this Gene ID
UniProt
View information about Cyclophilin D on UniProt.org

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Go To Top Version: 2    Revision Date: 09/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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