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Purified anti-Cytokeratin 17 Antibody

Pricing & Availability
Clone
W16131A (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type I cytoskeletal 17, keratin-17 (K-17), cytokeratin-17 (CK-17), KRT17, CK17, K17
Isotype
Rat IgG2b, κ
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Product Citations
publications
1_W16131A_PURE_Cytokeratin17_Antibody_WB_051017
Total cell lysates (15µg protein) from A549, A431 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5µg/mL purified anti- Cytokeratin 17 (clone W16131A) antibody, competitor’s antibody used at manufactures recommended concentration (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti- Cytokeratin 17 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and anti-GAPDH antibody. Lane M: Molecular Weight ladder.
  • 1_W16131A_PURE_Cytokeratin17_Antibody_WB_051017
    Total cell lysates (15µg protein) from A549, A431 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5µg/mL purified anti- Cytokeratin 17 (clone W16131A) antibody, competitor’s antibody used at manufactures recommended concentration (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti- Cytokeratin 17 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and anti-GAPDH antibody. Lane M: Molecular Weight ladder.
  • 2_W16131A_PURE_Cytokeratin17_2_041519
    A431 cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml rat IgG2b isotype control (top left), anti-Cytokeratin 17 (clone W16131A, top right), or competitor’s antibody used at manufactures recommended concentration (bottom left) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for anti- Cytokeratin 17 antibody, Alexa Fluor® 594 (red) conjugated donkey anti-rabbit IgG for competitor’s antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 3_W16131A_PURE_Cytokeratin17_3_072722.
    IHC staining using purified anti-Cytokeratin 17 (clone W16131A) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with (B) or without (A) 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scalebar = 50 μM
  • 4_W16131A_PURE_Cytokeratin17_4_072722
    IHC staining using purified anti-Cytokeratin 17 (clone W16131A) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with (B) or without (A) 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50
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697202 100 µg £189
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Description

Cytokeratin 17 (CK-17), also known as KRT17 or keratin-17 (K-17), is a type I intermediate filament protein. KRT expression is normally restricted to ectoderm-derived epithelial appendages such as thymus, glands, skin, hair, tooth, nail, and the endocervical mucosa. Cytokeratin-17 participates in promoting protein synthesis and cell growth through recruiting stratifin (SFN) to the cytoplasm and stimulating the Akt/mTOR pathway. Cytokeratin-17 was found to regulate chemokine expression, including the CXCR3 ligands CXCL9, CXCL10, and CXCL11. Cytokeratin 17 expression can be upregulated in response to chemical stimulation, wounding, and UV irradiation. Increased expression of Cytokeratin 17 is associated with lesion progression and poor prognosis in epithelial carcinoma. Cytokeratin 17 deficient mice show a delay in wound healing and attenuated tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Cytokeratin 17 peptide (415-432 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 2.0 µg per ml is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with mouse (in-house tested).

As per the Human Protein Atlas RNA level dataset, A549 cell is expected to have a low level of Cytokeratin 17 expression.

RRID
AB_2687136 (BioLegend Cat. No. 697202)

Antigen Details

Structure
432 amino acids with a predicted molecular weight of 48 kD. Belongs to the type-I intermediate filament family.
Distribution

Cytoplasm

Function
Cytokeratin 17 is a type I intermediate filament protein and is involved in promoting cell growth, chemokine production, and tumorigenesis.
Interaction
Forms a heterodimer with a type I or a type II keratin. Interacts with TRADD and SFN.
Biology Area
Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Hobbs RP, et al. 2016. Oncogene. 35:5653.
2. Escobar-Hoyos LF, et al. 2015. Cancer Res. 75:3650.
3. Chung BM, et al. 2015. J. Cell. Biol. 208:613.
4. Sankar S, et al. 2013. Mol. Cell. Biol. 33:4448.
5. Jin L, et al. 2014. Med. Res. Rev. 34:438.
6. Depianto D, et al. 2010. Nat. Genet. 42:910.

Gene ID
3872 View all products for this Gene ID
UniProt
View information about Cytokeratin 17 on UniProt.org
Go To Top Version: 2    Revision Date: 07/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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