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Purified anti-Cytokeratin 18 Antibody

Pricing & Availability
Clone
LDK18 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 18, Keratin type I cytoskeletal 18
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
LDK18_PURE_Cytokeratin18_Antibody_1_WB_062618
Whole cell lysates (15 µg protein) from A549, A431, HeLa, HEL (negative control) and U87-MG (negative control) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-Cytokeratin 18 antibody, clone LDK18. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • LDK18_PURE_Cytokeratin18_Antibody_1_WB_062618
    Whole cell lysates (15 µg protein) from A549, A431, HeLa, HEL (negative control) and U87-MG (negative control) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-Cytokeratin 18 antibody, clone LDK18. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • LDK18_PURE_Cytokeratin18_Antibody_2_ICC_062618
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL purified anti-cytokeratin 18 antibody (clone LDK18) for two hours at room temperature followed by Alexa Fluor® 594 conjugated goat anti-mouse IgG (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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689102 100 µg £150
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Description

Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues and is expressed at high levels in some tumor tissues. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been linked to cryptogenic cirrhosis. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3γ, 14-3-3ζ, 14-3-3σ, 14-3-3β, 14-3-3η, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human keratin 18 (CSETNDTKVLRH-COOH).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/ml (1:1000 - 1:4000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1 on formalin-fixed paraffin-embedded tissue (IHC-P).
 

This clone and BioLegend clone DA-7 displayed similar affinities when tested for western blot applications

Application References

(PubMed link indicates BioLegend citation)
  1. Hosoya A. et al. 2010. Anat. Rec. (Hoboken) 293:1864. (IHC-P)
RRID
AB_2629745 (BioLegend Cat. No. 689102)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
Distribution

Keratin 18 is widely expressed in normal single layer epithelial tissues (liver, pancreas) and is expressed at high levels in some tumor tissues.

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. USA 100:6063.
2. Kulesh DA, Oshima RG. 1989. Genomics 4:339.
3. Steinert PM, Roop DR. 1988. Ann. Rev. Biochem. 57:593.
4. Izawa I, et al. 2000. J. Biol. Chem. 275:34521.
5. Pankov R, et al. 1994. Proc. Natl. Acad. Sci. USA 91:873.

Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin 18 on UniProt.org
Go To Top Version: 3    Revision Date: 06/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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