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Purified anti-Cytoplasmic Dynein Intermediate Chain Antibody (Previously Covance catalog# MMS-400P)

Pricing & Availability
Clone
74.1 (See other available formats)
Regulatory Status
RUO
Other Names
Dynein light chain roadblock-type 1, Roadblock-1, ROBL/LC7-like 1, bithoraxoid-like protein, roadblock domain containing 1, dynein-associated protein Km23, cytoplasmic dynein light chain 2A, dynein light chain 2A, cytoplasmic
Previously
Covance Catalog# MMS-400P
Isotype
Mouse IgG2b, κ
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Product Citations
publications
74dot1_PURE_Cytoplasmic_Dynein_Int_Chain_Antibody_WB_112817
Western blot analysis of cell lysates from U87-MG, MCF-7 (Human) and Raw264.7 (Mouse) cells using Cytoplasmic Dynein Intermediate Chain Mouse primary antibody (clone , 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 74dot1_PURE_Cytoplasmic_Dynein_Int_Chain_Antibody_WB_112817
    Western blot analysis of cell lysates from U87-MG, MCF-7 (Human) and Raw264.7 (Mouse) cells using Cytoplasmic Dynein Intermediate Chain Mouse primary antibody (clone , 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 74dot1_PURE_Cytoplasmic_Dynein_Int_Chain_Antibody_WB_2_112817
    Immunofluorescence of HeLa cells with (A) mouse IgG2b, κ isotype control (Negative, Cat. No. 401202) or (B-D) Cytoplasmic Dynein Intermediate Chain antibody. Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/15. Concentrations for (A, B) is 10 µg/ml, (C) is 4 µg/ml and (D) is 2 µg/ml.
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904901 100 µL £174
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Description

Dyneins are motor proteins that transport various cellular cargo along cytoskeletal microtubules in eukaryotic cells. Dynein has the same basic structures in all cell types and is composed of two or three distinct heavy chains (450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD).  The cytoplasmic dynein intermediate chain is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against purified bovine brain cytoplasmic dynein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml (1:1000). For immunocytochemistry, a concentration range of 4.0 - 10.0 μg/ml (1:100-1:250) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP).

*Predicted MW = 74 kD. This antibody is known not to react in squid and nematode.

Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.

Application References

(PubMed link indicates BioLegend citation)
  1. Miller MG, et al. 1999. Biol Reprod. 60:1047.
  2. Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141.
  3. Pfister KK, et al. 1996.J Biol Chem. 271:1687.
  4. Dillman JF, et al. 1994. J Cell Biol. 127:1671.
  5. Combs J, et al. 2006. PNAS. 103:14883.
  6. Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed
  7. Burakov A, et al. 2008. Traffic. 9:472.
  8. Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed
  9. Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed
Product Citations
  1. Dwivedi D, et al. 2019. J Cell Biol. 218:871. PubMed
  2. Payne C, et al. 2003. J Cell Sci. 116:4727-4738. PubMed
  3. Chen J, et al. 2005. J Cell Biol. 169:383-389. PubMed
  4. Sabblah TT, et al. 2018. Sci Rep. 1.540972222. PubMed
  5. Nath S, et al. 2016. J Immunol. 197: 2090 - 2101. PubMed
  6. Zhang J, et al. 2016. Sci Rep. 6:37050. PubMed
  7. Semenova I, et al. 2014. Mol Biol Cell. 25:3119-3132. PubMed
RRID
AB_2565044 (BioLegend Cat. No. 904901)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
83658 View all products for this Gene ID
UniProt
View information about Cytoplasmic Dynein Intermediate Chain on UniProt.org
Go To Top Version: 6    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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