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Purified anti-Daxx Antibody

Pricing & Availability
Clone
P87A11 (See other available formats)
Regulatory Status
RUO
Other Names
DAP6, EAP1, BING2, Death-Domain Associated Protein, Fas-Binding Protein, CENP-C Binding Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P87A11_PURE_Daxx_Antibody_1_WB_012617_UPDATED
Total cell lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2), THP1 (lane 3), A549 (lane 4) and NIH3T3 (lane 5) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Daxx antibody, clone P87A11 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Daxx antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
  • P87A11_PURE_Daxx_Antibody_1_WB_012617_UPDATED
    Total cell lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2), THP1 (lane 3), A549 (lane 4) and NIH3T3 (lane 5) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Daxx antibody, clone P87A11 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Daxx antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
  • P87A11_PURE_Daxx_Antibody_2_IF_100716
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for ten minutes and then permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. Cells were intracellularly stained with 5 µg/mL anti-Daxx (clone P87A11) in blocking buffer overnight at 4°C followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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691402 100 µg £174
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Description

Daxx is a multifunctional protein that interacts with a wide variety of proteins. It was originally identified as a signaling protein that binds to the Fas death domain, enhances Fas-mediated apoptosis and activates the JNK pathway. Later, Daxx was found to be involved in gene regulation. It inhibits the activation of some sumoylated transcription factors such as Pax3 and Ets-1 in the nucleus. When recruited to promyelocytic leukemia protein nuclear bodies (PML-NB) through interaction with PML, its transcription repressor activity is alleviated.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human Daxx (aa 541-720) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The predicted molecular weight of Daxx is 83 kD. The observed molecular weight is around 110 kD on Western blot due to post-translational hyperphosphorylation. Clone P87A11 does not cross-react with mouse (in-house tested).

RRID
AB_2632649 (BioLegend Cat. No. 691402)

Antigen Details

Structure
740 amino acids with a predicted molecular weight of 83 kD. Contains a SUMO interaction motif at C-terminal region.
Distribution

Mainly located in the nucleus. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress.

Function
Enhance Fas-mediated apoptosis. Involve in regulation of gene transcription.
Interaction
Daxx is found to have interactions with several different proteins, such as Pax3, CENP-C, DNA methyltransferase I, MCSR1, Fas, STAT3 and promyelocytic leukemia protein (PML).
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Yang X, et al. 1997. Cell. 89:1067-76.
2. Schreiner S, Wodrich H. 2013. J. Virol. 87:10412-22.
3. Li H, et al. 2000. Mol. Cell. Biol. 20:1784-96.
4. Takahashi Y, et al. 2004. Oncogene. 23:2819.

Gene ID
1616 View all products for this Gene ID
UniProt
View information about Daxx on UniProt.org
Go To Top Version: 3    Revision Date: 08/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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