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Purified anti-DDX5 Antibody

Pricing & Availability
Clone
14B9A58 (See other available formats)
Regulatory Status
RUO
Other Names
Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
14B9A58_DDX5_Purified_1_WB_062518
Whole cell lysates (15 µg protein) from HeLa, HEL, MOLT-4, and RAW264.7 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-DDX5 antibody, clone 14B9A58. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • 14B9A58_DDX5_Purified_1_WB_062518
    Whole cell lysates (15 µg protein) from HeLa, HEL, MOLT-4, and RAW264.7 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-DDX5 antibody, clone 14B9A58. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • 14B9A58_DDX5_Purified_2_WB_062518
    Total cell lysate from 293T cells (lane 1) or NIH-3T3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX5 (clone 14B9A58) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 14B9A58_DDX5_Purified_3_ICC_062518
    HeLa cells were stained with purified anti-DDX5 (14B9A58) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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661302 100 µg £182
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Description

DDX5, also named RNA helicase p68, is a prototypic member of the DEAD (Asp-Glu-Ala-Asp) box family. DDX5 has been shown to be involved in the processes requiring the alteration of RNA structures, such as the maturation of pre-mRNA, rRNA, and miRNA. DDX5 also plays important helicase-independent roles in transcriptional regulation. DDX5 can act as a transcriptional coactivator of the androgen receptor (AR), estrogen receptor α (ERα), tumor suppressor p53, MyoD, RUNX2, and β-catenin, which are involved in the regulation of cell proliferation, differentiation, and tumorigenesis. DDX5 has been shown to be up-regulated in various cancers, including breast cancer, prostate cancer, and colorectal cancer, suggesting that DDX5 may participate in tumor development. DDX5 can also act as a transcriptional repressor through interacting with HDAC1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX5 recombinant protein (482-614 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2563942 (BioLegend Cat. No. 661302)

Antigen Details

Structure
614 amino acids, predicted molecular weight of 69 kD. Contains a DEAD box domain, a helicase domain, and a transactivation domain.
Distribution

Nucleus.

Function
DDX5 is an ATP-dependent RNA helicase, regulating RNA processing and ribosome biogenesis. DDX5 is also involved in transcription by acting as a transcriptional coactivator or a repressor.
Interaction
DDX5 interacts with DDX17, RBM4, AGO1, AGO2, ESR1, p300, RUNX2, and HDAC1.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Iyer RS, et al. 2014. Cell Cycle. 13:1413.
2. Samaan S, et al. 2014. Nucleic Acids Res. 42:2197.
3. Fuller-Pace FV. 2013. Biochim. Biophys. Acta. 1829:756.
4. Wang D, et al. 2012. Mol. Cell Proteomics. 11:M111.011932.
5. Saporita AJ, et al. 2011. Cancer Res. 71:6708.
6. Clark EL, et al. 2008. Cancer Res. 68:7938.

Gene ID
1655 View all products for this Gene ID
UniProt
View information about DDX5 on UniProt.org
Go To Top Version: 3    Revision Date: 06/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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