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Purified anti-DEPTOR Antibody

Pricing & Availability
Clone
W17128A (See other available formats)
Regulatory Status
RUO
Other Names
DEPTOR, DEPDC6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17128A_Purified_DEPTOR_Antibody_052219_updated.png
Total cell lysates (15 µg total protein) from A431, NTERA2 (negative controls), Daudi, RPMI-8226 (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL Purified anti-DEPTOR Antibody, clone W17128A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W17128A_Purified_DEPTOR_Antibody_052219_updated.png
    Total cell lysates (15 µg total protein) from A431, NTERA2 (negative controls), Daudi, RPMI-8226 (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL Purified anti-DEPTOR Antibody, clone W17128A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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610201 25 µg £70
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610202 100 µg £174
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Description

DEPTOR/DEPDC6, found only in vertebrates, plays an important role in multiple cellular processes such as cell growth, autophagy, apoptosis, ER stress and adipogenesis. It inbibits mTORC1 and mTORC2 kinase activity through direct interaction with mTOR in these complexes. DEPTOR also associates with chromatin and may regulate transcription, and can act as either an oncogene or oncosuppressor depending on cell- or tissue-specific context.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human DEPTOR Protein (1-219 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783359 (BioLegend Cat. No. 610201)
AB_2783359 (BioLegend Cat. No. 610202)

Antigen Details

Distribution

Cytoplasm/ Nucleus, A little bit more in Cytoplasm

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Signal Transduction, Transcription Factors
Molecular Family
Tumor Suppressors
Gene ID
64798 View all products for this Gene ID
UniProt
View information about DEPTOR on UniProt.org
Go To Top Version: 1    Revision Date: 05/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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