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Purified anti-Desmoglein 3 Antibody

Pricing & Availability
Clone
DSG3/5H10 (See other available formats)
Regulatory Status
RUO
Other Names
DSG3, PVA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DSG3-5H10_PURE_Desmoglein3_Antibody_1_090220
Western blot of purified anti-Desmoglein 3 (clone DSG3/5H10). Lane 1: Molecular weight marker; Lane 2: 5 µg of HaCaT cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending DSG3 expression; predicted expression data was obtained from Human Protein Atlas.
  • DSG3-5H10_PURE_Desmoglein3_Antibody_1_090220
    Western blot of purified anti-Desmoglein 3 (clone DSG3/5H10). Lane 1: Molecular weight marker; Lane 2: 5 µg of HaCaT cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending DSG3 expression; predicted expression data was obtained from Human Protein Atlas.
  • DSG3-5H10_PURE_Desmoglein3_Antibody_2_090220
    ICC staining of purified anti-Desmoglein 3 (clone DSG3/5H10) on HaCaT cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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871901 25 µg £73
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871902 100 µg £189
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Description

Desmoglein-3 is a glycoprotein mainly expressed in the basal lower layers of the epidermis and on mucosal surfaces. It is the Ca2+ binding transmembrane components of the desmosomes that functions as cell-cell junctions between epithelial cells as well as other cell types. Clinically, desmoglein-3 has been identified as one of the autoantigens in the autoimmune skin blistering disease pemphigus vulgaris. It also serves as a prognostic marker of esophageal squamous cell carcinoma. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Wan H, et al. 2016. Data Brief. 6:124-34 (WB, ICC)
  2. Walter E, et al. 2017. Sci Rep. 7:3579 (ICC)
  3. Tsang SM, et al. 2010. PloS One. 5:e14211 (WB, ICC)
RRID
AB_2876750 (BioLegend Cat. No. 871901)
AB_2876750 (BioLegend Cat. No. 871902)

Antigen Details

Structure
Desmoglein 3 is a 999 amino acid protein with predicted molecular mass of 108 kD and observed molecular mass of ~130 kD.
Distribution

Tissue Distribution: Esophagus, skin, tonsil
Cellular Distribution: Plasma membrane, cytosol, extracellular

Function
Desmoglein-3 is the Ca2+ binding transmembrane components of the desmosomes that functions as cell-cell junctions between epithelial cells as well as other cell types.
Interaction
PKP3, JUP, WWOX, FRMD1, OAZ3
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Adaptive Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family
Adhesion Molecules, Gap Junction Proteins
Antigen References
  1. Mahmoudi H, et al. 2020. Int J Immunogenet. 47:41-9
  2. Spindler V & Waschke J. 2018. Front immunol. 9:136
  3. Pan M, et al. 2015. J Dermatol. 42:11-7
Gene ID
1830 View all products for this Gene ID
UniProt
View information about Desmoglein 3 on UniProt.org
Go To Top Version: 1    Revision Date: 09/02/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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