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Purified anti-DNA-PKcs Phospho (Thr2609) Antibody

Pricing & Availability
Clone
10B1 (See other available formats)
Regulatory Status
RUO
Other Names
DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10B1_PURE_DNA-PKcs_Antibody_1_IF_012316
3 minutes UV treated HeLa cells (recovered 30 minute) were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
  • 10B1_PURE_DNA-PKcs_Antibody_1_IF_012316
    3 minutes UV treated HeLa cells (recovered 30 minute) were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
  • 10B1_PURE_DNA-PKcs_Antibody_2_IF_012316
    Untreated HeLa cells were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
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612901 25 µg £81
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612902 100 µg £201
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Description

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide
Formulation
This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Reindl J, et al. 2022. Sci Rep. 12:1305. PubMed
  2. Eid M, et al. 2014. J Immunol. 192:5529. PubMed
RRID
AB_315784 (BioLegend Cat. No. 612901)
AB_315784 (BioLegend Cat. No. 612902)

Antigen Details

Structure
PI3/PI4-Kinases family. Spliced products 460 kD, 470 kD
Distribution

Nuclear

Function
Serine/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator
Interaction
Ku70/Ku80, KIP, DNA-ligase IV, XRCC4
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Hartley K, et al. 1995. Cell. 82:849.
2. Connelly M, et al. 1996. Gene. 175:271.
3. Douglas P, et al. 2002. Biochem. J. 368:243
4. Calsou P, et al. 2003. J. Mol. Biol. 326:93.

Regulation
Must bind DNA ends to become active
Gene ID
5591 View all products for this Gene ID
UniProt
View information about DNA-PKcs Phosphorylated on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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