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Purified anti-Doublecortin (DCX) Antibody

Pricing & Availability
Clone
O94A3 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O94A3_Purified_Doublecortin_Antibody_071819
Western blot of purified anti-Doublecortin (DCX) antibody (clone O94A3). Lane 1: Molecular weight marker; Lane 2: 10 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O94A3_Purified_Doublecortin_Antibody_071819
    Western blot of purified anti-Doublecortin (DCX) antibody (clone O94A3). Lane 1: Molecular weight marker; Lane 2: 10 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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868501 25 µg £73
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868502 100 µg £189
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Description

Doublecortin (DCX) is a microtubule-associated protein that is predominantly expressed in embryonic brains by neuronal precursor cells or immature neurons. DCX is also expressed in the adult brains in regions such as subventricular zones and dentate gyrus, where neuronal precursor cells are localized, and thus has been widely used as a marker for neurogenesis. Doublecortin binds to the microtubule cytoskeleton and stabilizes microtubule bundles. Mutation in DCX has been identified in diseases such as X-linked lissencephaly and double cortex syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human recombinant DCX protein produced in HEK293T cell.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814620 (BioLegend Cat. No. 868501)
AB_2814620 (BioLegend Cat. No. 868502)

Antigen Details

Structure
DCX is a 366 amino acid protein with predicted and observed molecular mass of ~41 kD
Distribution

Tissue Distribution: Brain
Cellular Distribution: Cytoskeleton, cytosol, and nucleus

Function
DXC is a markers for neurogenesis. It binds to the microtubule cytoskeleton and stabilizes microtubule bundles.
Interaction
Tubulin, USP9X
Cell Type
Neural Stem Cells, Neurons
Biology Area
Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, Microtubules
Antigen References
  1. Ayanlaja AA, et al. 2017. Front Mol Neurosci. 10:199
  2. Reiner O. 2013. Scientifica (Cairo). 2013:393975
  3. Reiner O, et al. 2004. Cell Cycle. 3(6):747
Gene ID
13193 View all products for this Gene ID
UniProt
View information about Doublecortin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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