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Purified anti-DYKDDDDK Epitope Tag Antibody (Previously Covance catalog# PRB-132P)

Pricing & Availability
Clone
Poly9024 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG, FLAG tag
Previously
Covance Catalog# PRB-132P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Total cell lysates (15 µg protein) from 293E (lane 1) and 293E cells expressing RORγ-DYKDDDDK (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:1000 dilution) of Purified anti-DYKDDDDK Epitope Tag Antibody, clone Poly9024 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-rabbit-IgG secondary antibody conjugated to HRP for Purified anti-DYKDDDDK Epitope Tag Antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • Total cell lysates (15 µg protein) from 293E (lane 1) and 293E cells expressing RORγ-DYKDDDDK (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:1000 dilution) of Purified anti-DYKDDDDK Epitope Tag Antibody, clone Poly9024 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-rabbit-IgG secondary antibody conjugated to HRP for Purified anti-DYKDDDDK Epitope Tag Antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • Purified_DYKDDDDK_Antibody_IHC_021315
    Identification of overexpressed DYKDDDDK-p29 protein in HeLa cells (48 hours after transfection). Anti-DYKDDDDK was used at 1:200 and was detected using a goat anti-rabbit AlexFluor 594 at 1:800. Photo courtesy of J. Lui.
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902401 200 µL £295
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and immunocytochemistry. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody DYKDDDDK was raised against the sequence CDYKDDDDK.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Western Blotting (WB) and Immunocytochemistry (ICC) of tagged proteins.

This antibody recognizes the FLAG® epitope tag. The DYKDDDDK antibody does not require Ca++ for binding. It reacts with both N-terminal and C-terminal fusion proteins.

This product may contain other non-IgG subtypes

Product Citations
  1. Trothen SM, et al. 2022. FEBS Lett. 596:232. PubMed
  2. Rogers EJ, et al. 2019. Front Mol Neurosci. 12:43. PubMed
  3. Li Y, et al. 2008. J Biol Chem. 283:22443-22456. PubMed
RRID
AB_2565020 (BioLegend Cat. No. 902401)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org
Go To Top Version: 5    Revision Date: 01/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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