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Purified anti-Dynamin-1 Antibody

Pricing & Availability
Clone
P83G4B6 (See other available formats)
Regulatory Status
RUO
Other Names
DNM1, DNM, DMN-1, dynamin 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P83G4B6_PURE_Dynamin1_Antibody_1_091417
Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_1_091417
    Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_2_091417
    Total lysates (15 µg protein) from human brain, mouse brain and rat brain were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody. 1:1000 (0.5 micro;g/mL) dilution of β-actin (2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_3_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
  • P83G4B6_PURE_Dynamin1_Antibody_4_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
  • P83G4B6_PURE_Dynamin1_Antibody_5_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
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604001 25 µg £70
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604002 100 µg £174
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Description

Dynamin-1, also known as DMN-1, is a 100 kD protein with GTPase activity. Dynamin-1 is involved in several intracellular trafficking processes including synaptic vesicle recycling, neurotransmitter reuptake and receptor internalization.  In addition, Dynamin-1 may be necessary for the establishment and proper maintenance of mature neuronal structure. Studies have shown that Dynamin-1 is upregulated during new neurite formation. Conversely, Dynamin-1 is downregulated during neurite retraction. Silencing the initiation codon for Dynamin limits the formation of axon-like structures.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Dynamin-1 (515-750a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 – 2 µg/mL). For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1:100-1:250 diluted (2 – 5 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2716194 (BioLegend Cat. No. 604001)
AB_2716194 (BioLegend Cat. No. 604002)

Antigen Details

Structure
864 amino acids with a predicted molecular weight of 97 kD.
Distribution

Cytoplasm

Function
Involved in producing microtubule bundles and able to bind and hydrolyze GTP
Interaction
Interacts with CAV1, SH3GLB1, SH3GL1, SH3GL2, SH3GL3, PHOCN, PACSIN1, PACSIN2, PACSIN3, SNX9, MYO1E, SNX33, UNC119 and DIAPH1.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Obar RA, et al. 1990. Nature. 347:256.
  2. Chen-Hwang MC, et al. 2002. J Biol Chem. 277:17597.
  3. Grabs D, et al. 1997. J Biol Chem. 272:13419.
  4. Wunderlich L, et al. 1999. Cell. Signal. 11:25.
Gene ID
1759 View all products for this Gene ID
UniProt
View information about Dynamin-1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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