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Purified anti-Dynamin-2 Antibody

Pricing & Availability
Clone
W22024F (See other available formats)
Regulatory Status
RUO
Other Names
DYN2, DI-CMTB, CMTDIB, CMTDI1, DYNII, CMT2M, Dynamin II, LCCS5, Dynamin 2, DNM2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22024F_PURE_Dynamin-2_WB_021424
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-Dynamin-2 (clone W22024F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22024F_PURE_Dynamin-2_WB_021424
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-Dynamin-2 (clone W22024F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22024F_PURE_Dynamin-2_ICC_021424
    HeLa cells (mock) (panels A and B) and Dynamin-2 knockdown cells (KD) (panels C and D) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature, respectively. Cells were then stained with Purified anti-Dynamin-2 (clone W22024F), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panels B and D). The image was captured with a 40X objective. Scale bar: 25 µm
  • C. W22024F_PURE_Dynamin-2_IHCP_021424
    IHC staining using Purified anti-Dynamin-2 (clone W22024F) on paraffin-embedded human colon (panel A) and human kidney (panel B). Following antigen retrieval using 1X Citrate buffer (Cat. No. 420901) or Tris-EDTA pH 9.0, respectively. The tissue sections were incubated with 1 µg/mL of Purified anti-Dynamin-2 (clone W22024F) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were captured with a 40X objective. Scale bar: 50 µm
  • D. W22024F_PURE_Dynamin-2_ICFC_021424
    293T cells which were untransfected (filled histogram) or transfected with a knockdown shRNA against Dynamin-2 (open histogram) were fixed with Fixation Buffer (Cat. No. 420801) then permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401). Cells were then stained with either Purified anti-Dynamin-2 (solid line histograms) or purified rat IgG2a, κ isotype control (Cat. No. 400502) (dashed line, open histogram), followed by incubation with 0.1 µg/test of APC Goat anti-rat IgG (Cat. No. 405407) for 15 min at room temperature.
Cat # Size Price Quantity Check Availability Save
633151 25 µg £120
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633152 100 µg £301
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Description

Dynamin-2 (DNM2) belongs to the dynamin family. It consists of GTPase domain (G-domain) at the N-terminus, middle domain, the lipophilic pleckstrin homology [PH] domain, GTPase effector domain (GED), and proline rich domain (PRD) at the C-terminus. The PH domain interacts with membrane phosphoinositides, while PRD domain interacts with SH3 domain-containing proteins. Dynamin-2 is a large mechanochemical GTPase involved in various cellular processes such as endocytosis, apoptosis, cytokinesis, phagocytosis, neuronal morphology, axonal growth, cell migration, cell membrane trafficking and cytoskeletal regulation. The studies indicate that mutations in Dynamin-2 cause centronuclear myopathy (CNM), Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB), hereditary spastic paraplegia and lethal congenital contractures syndrome type 5 (LCCS5). Additionally, numerous studies have implicated Dynamin-2 overexpression in several types of cancer and metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Dynamin-2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 0.2 - 5.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0625 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone does not cross-react with mouse in western blotting (WB).

For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with ice cold-methanol, or ice-cold methanol fixation.

For immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.

For intracellular staining for flow cytometry (ICFC), the recommended dosage is 0.0625 μg per test using intracellular staining buffer (Cat. No. 421002), Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), True-Phos™ Perm Buffer (Cat. No. 425401) and True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for WB, ICC and ICFC.

RRID
AB_3097580 (BioLegend Cat. No. 633151)
AB_3097580 (BioLegend Cat. No. 633152)

Antigen Details

Structure
Dynamin-2 protein is a 870 amino acid protein with a predicted molecular weight of 98 kD.
Distribution

Ubiquitously expressed

Function
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Dynamin-2 is also involved in cytokinesis.
Interaction
MYOF, CTTN, ACTN1, SHANK1, SHANK2, SH3BP4 and NOSTRIN, SNX9, SNX18, SNX33, MYO1E, PSTPIP1, CTNND2, PIK3C3, BIN1
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Cao H, et al. 1998. Mol Biol Cell. 9:2595-609.
  2. Gu C, et al. 2010. EMBO J. 29:3593-606.
  3. Bitoun M, et al. 2006. Med Sci. 22:101-2.
  4. Sidiropoulos P.N.M, et al. 2012. Brain. 135:1395-411.
  5. Sambuughin N, et al. 2015. BMC Neurol. 15:223.
  6. Koutsopoulos OS, et al. 2012. European Journal of Human Genetics. 21:637.
  7. Trochet D, et al. 2021. J Exp Clin Cancer Res. 40:238.
Gene ID
1785 View all products for this Gene ID
UniProt
View information about Dynamin-2 on UniProt.org
Go To Top Version: 1    Revision Date: 02/14/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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