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Purified anti-E. coli RNA Polymerase β Prime Antibody

Pricing & Availability
Clone
NT73 (See other available formats)
Regulatory Status
RUO
Other Names
RNA pol beta prime, rpoC, tabB, DNA-directed RNA polymerase subunit beta prime, RNAP subunit beta prime, RNA polymerase subunit beta prime, Transcriptase subunit beta prime
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
NT73_PURE_E.coliRNAPolymeraseBetaPrime_Antibody_WB_102717_updated
Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase β Prime antibody (clone NT73). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • NT73_PURE_E.coliRNAPolymeraseBetaPrime_Antibody_WB_102717_updated
    Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase β Prime antibody (clone NT73). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • NT73_PURE_E.coliRNAPolymeraseBetaPrime_Antibody_WB_2_061418_updated
    Total cell lysate from Hela cells (lane 1) and E Coli. Strain DH5α (lane 2) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with a 1:1000 dilution (1 µg/mL) of purified anti-E. coli RNA Polymerase β Prime antibody (clone NT73) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for anti-E. coli RNA Polymerase β Prime Antibody (upper blot). Ponceau S staining (lower blot) was used as loading control. Lane M: Molecular Weight Marker
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662904 100 µg £221
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
1295-1417 (SLAELLNAGLGGS) of the E. coli RNA polymerase β subunit.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone NT73 is extremely useful for purifying holoenzymes from enterics. It does not cross-react with Mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. Clone NT73 recognizes epitope 1295-1417 (SLAELLNAGLGGS).

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 1992. Biochemistry 31:7003.
  2. Thompson NE, et al. 2003. Anal. Biochem. 323:171.
Product Citations
  1. Bray AS, et al. 2022. MBio. 13:e0359521. PubMed
RRID
AB_2566242 (BioLegend Cat. No. 662904)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Hurwitz J, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta Prime on UniProt.org
Go To Top Version: 6    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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