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Purified anti-EGF Antibody

Pricing & Availability
Clone
M1401E03 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor, Urogastrone (URG), HOMG4
Isotype
Mouse IgG1, κ
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Product Citations
publications
M1401E03_Purified_EGF_Antibody_IP_082415
Immunoprecipitation/Western blot analysis of 200ng recombinant EGF. Lane 1 was immunoprecipitated with mouse IgG control antibody, lane 2 was immunoprecipitated with EGF antibody (clone M1401E03), and lane 3 was loaded with 25ng recombinant human EGF. Western blot analysis was performed using goat anti-human EGF antibody followed by HRP-anti-goat IgG.
  • M1401E03_Purified_EGF_Antibody_IP_082415
    Immunoprecipitation/Western blot analysis of 200ng recombinant EGF. Lane 1 was immunoprecipitated with mouse IgG control antibody, lane 2 was immunoprecipitated with EGF antibody (clone M1401E03), and lane 3 was loaded with 25ng recombinant human EGF. Western blot analysis was performed using goat anti-human EGF antibody followed by HRP-anti-goat IgG.
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679502 100 µg £197
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Description

Epidermal growth factor (EGF) is a small 6 kD polypeptide and has six conserved cysteine residues that form three intramolecular disulfide bonds. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF/EGF receptor signaling pathway plays an important role in proliferation, differentiation, and migration of a variety of cell types, especially in epithelial cells. The binding of EGF to EGFR induces receptor dimerization, which is required for activating the tyrosine kinase dimerization in the receptor cytoplasmic domain. In addition, the binding of EGF to its receptor triggers several signal transduction pathways including JAK/STAT, Ras/ERK/, and PI3K/AKT pathways. The blocking of the EGF/EGFR pathway can suppress some tumor cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human EGF, amino acids Asn971 - Arg1023, with an N-terminal Met (Accession# P01133) was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IP - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
RRID
AB_2566190 (BioLegend Cat. No. 679502)

Antigen Details

Structure
54 amino acids wtih a predicted molecular weight of approximately 6 kD.
Distribution

Mammary gland cells, macrophages, gut epithelial cells, cells in the nervous system, and the kidney.

Function
EGF is a potent mitogen for many cells in culture, and in vivo, it induces the proliferation and the differentiation of the skin, cornea, lungs, trachea, and among other tissues. Processing of pro EGF in different tissues is not equally efficient. The precursor is processed to mature EGF in the submaxillary glands, pancreas, small intestine, and the mammary glands. EGF is fully processed, stored in secretory granules, and secreted in saliva. In the kidney, EGF is present in unprocessed or intermediated forms on the cell surface.
Interaction
EGFR, RHBDF2, RHBDF1, and ERAD.
Ligand/Receptor
EGFR.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Henson ES and Gibson SB. 2006. Cell Signal. 18:2089.
2. Burgess AW, et al. 2003. Mol. Cell. 12:541.
3. Imai Y, et al. 1982. Cancer Res. 42:4394.
4. Barnes DW. 1982. J. Cell. Biol. 93:1.
5. Heo JS, et al. 2006. Am. J. Physiol. Cell Physiol. 290:C123.
6. Bodnar RJ. 2013. Adv. Wound Care (New Rochelle). 2:24.
7. Zeng F, et al. 2014. Semin. Cell Dev. Biol. 28:2.

Gene ID
1950 View all products for this Gene ID
UniProt
View information about EGF on UniProt.org
Go To Top Version: 2    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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