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Purified anti-eIF3I (p36) Antibody

Pricing & Availability
Clone
17C11 (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic translation initiation factor 3 subunit I, Eukaryotic translation initiation factor 3 subunit 2, eIF-3-beta, eIF3 p36, TGF-beta receptor-interacting protein 1, TRIP-1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
17C11_PURE_eIF3I_Antibody_WB_122117
Total lysates (15 µg protein) from Ramos (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-eIF3I (p36) antibody, clone 17C11 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-eIF3I (p36) antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder
  • 17C11_PURE_eIF3I_Antibody_WB_122117
    Total lysates (15 µg protein) from Ramos (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-eIF3I (p36) antibody, clone 17C11 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-eIF3I (p36) antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder
Cat # Size Price Quantity Check Availability Save
646701 25 µg £85
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646702 100 µg £182
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Description

The elF3I (eIF3-p36) is a subunit of eukaryotic translation initiation factor 3 (eIF3) complex that plays a central role in the protein translation initiation. The eIF3 consists of at least 10 subunits ranging from 35 to 170 kd. elF3-p36 is constitutively expressed in many tissues, including spleen thymus, prostate, colon, ovary, etc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 to 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Panthu B, et al. 2020. J Biol Chem. 295:1843. PubMed
  2. Jeong SJ, et al. 2019. Nat Commun. 10:1357. PubMed
RRID
AB_1732022 (BioLegend Cat. No. 646701)
AB_1732022 (BioLegend Cat. No. 646702)

Antigen Details

Distribution

Expressed in most tissues, including spleen, thymus, prostate, ovary, etc.

Function
Translation initiation.
Biology Area
Cell Biology, Protein Synthesis
Molecular Family
Nuclear Markers
Antigen References

1. Tymms MJ. et al. 1997. Oncogene 15:2449
2. H TK. et al. 2000 Blood 96:747

Gene ID
8668 View all products for this Gene ID
UniProt
View information about eIF3I on UniProt.org
Go To Top Version: 2    Revision Date: 05/11/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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