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Purified anti-EphB4 Antibody

Pricing & Availability
Clone
W20295K (See other available formats)
Regulatory Status
RUO
Other Names
Ephrin type-B receptor 4 (EphB4), HTK, MYK1, TYRO11
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W20295K_PURE_EphB4_WB_071423
Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-EphB4 (clone W20295K) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W20295K_PURE_EphB4_WB_071423
    Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-EphB4 (clone W20295K) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W20295K_PURE_EphB4_ICC_071423
    Jurkat cells (negative cell line, panels A and B) and HeLa cells (positive cell line, panels C and D) were fixed and permeabilized with 100% ice-old methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5 μg/mL of purified anti-EphB4 (clone W20295K), followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801, panels A and C). Images were captured with a 60X objective. Scale bar: 50 µm
  • C. W20295K_PURE_EphB4_FC_071423
    MCF-7 cells were stained with purified anti-EphB4 (clone W20295K) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
Cat # Size Price Quantity Check Availability Save
619151 25 µg £79
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619152 100 µg £257
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Description

Ephrin type-B receptor 4 (EphB4) is a member of the Eph family of receptor tyrosine kinases that bind to the Ephrin family of ligands. Specifically, EphB4 binds to Ephrin B2, in a process involving direct cell-to-cell interaction, to initiate signaling pathways that influence cell behavior such as differentiation, adhesion and migration.  EphB4 has been shown to be involved in heart morphogenesis and angiogenesis, and in the tumorigenesis of cancers such as gliomas, breast and prostate cancer. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human EphB4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in IHC-P.

This product does not recognize mouse EphB4 in WB.

For ICC, we recommend fixation/permeabilization with 100% ice-cold methanol. We do not recommend using 4% PFA as a fixative.

RRID
AB_3068204 (BioLegend Cat. No. 619151)
AB_3068204 (BioLegend Cat. No. 619152)

Antigen Details

Structure
EphB4 is a 987 amino-acid protein with a predicted molecular weight of 108.3 kD.
Distribution

Plasma membrane

Function
Receptor tyrosine kinase
Ligand/Receptor
Ephrin B2
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Signal Transduction
Antigen References
  1. Piffko A, et al. 2022. Int J Mol Sci. 23:1679. 
  2. Chen Y, et al. 2017. Semin. Cancer Biol. 56:37-46.
  3. Du E, et al. 2020. Mol Biol Rep. 4:4681-4690.
Gene ID
2050 View all products for this Gene ID
UniProt
View information about EphB4 on UniProt.org
Go To Top Version: 1    Revision Date: 07/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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