- Clone
- Poly21A07 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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604951 | 50 µL | £121 | ||||
604952 | 200 µL | £241 |
ERK1/2 (extracellular signal-regulated protein kinase) are members of the mitogen-activated protein kinase (MAPK) family of serine/threonine kinases. ERK1, also called Mitogen-activated protein kinase 3 (MAPK3) and ERK2, also called Mitogen-activated protein kinase 1 (MAPK1), are both integral effectors of the Ras-dependent extracellular signal-regulated kinase 1 (ERK1/2) mitogen-activated (MAP) kinase pathway. As such, they are vital regulators of cell cycle, differentiation, proliferation, stress-response, survival, and senescence. ERK1/2 are activated via phosphorylation of their tyrosine (Tyr204) and threonine (Thr202) residues by MEK1 and MEK2 in response to mitogenic stimuli. Upon activation, ERK1/2 translocate to the nucleus where they function in the regulation of transcription and translation. Dysregulation of ERK1/2 and the hyper-activation of the ERK pathway are characteristics of many cancers where it contributes to tumorigenesis, metastasis, and treatment-resistance, making ERK1/2 an important therapeutic target.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Synthetic peptide of human Erk1/2 phosphorylated at Thr202/Tyr204
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Use 20 μL per 10 mL antibody dilution buffer for each gel.
- Application Notes
-
This clone was tested by ICC in HeLa cells treated with or without 100 ng/mL PMA. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. All three methods resulted in weak staining and a lack of phospho-specificity.
- RRID
-
AB_2927813 (BioLegend Cat. No. 604951)
AB_2927813 (BioLegend Cat. No. 604952)
Antigen Details
- Structure
- ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD. They share 85% homology.
- Distribution
-
Ubiquitously expressed/ Localized to the cell membrane and nucleus
- Function
- Serine/threonine-protein kinase, phosphatase
- Interaction
- Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Busca R, et al. 2016. Front. Cell Dev. Biol. 4:53.
- Meloche S and Pouyssegur J. 2007. Oncogene. 26:3227.
- Wortzel I and Seger R. 2011. Genes and Cancer. 3:195.
- Gene ID
- 5594 View all products for this Gene ID 5595 View all products for this Gene ID
- UniProt
- View information about ERK1/2 on UniProt.org
Related FAQs
Other Formats
View All ERK1/2 Phospho (Thr202/Tyr204) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ERK1/2 Phospho (Thr202/Tyr204) | Poly21A07 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-ERK1/2 Phospho (Thr202/Tyr204)
Whole cell extracts (15 μg protein) from serum-starved Jurka... Whole cell extracts (15 μg protein) from serum-starved Raw26...
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