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Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
Poly21A07 (See other available formats)
Regulatory Status
RUO
Other Names
Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly21A07_PURE_ERK1-2-Phospho_WB_112922
Whole cell extracts (15 μg protein) from serum-starved Jurkat cells untreated (-) or treated (+) with 100 ng/mL PMA for 5 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone Poly21A07) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG (Cat. No. 410406) at a 1:3000 dilution. Equal ERK1/2 loading was confirmed by probing membranes with purified anti-ERK1/2 (clone W15133A) (Cat. No. 687002) at 1.0 µg/mL. Lane M: Molecular weight marker
  • A_Poly21A07_PURE_ERK1-2-Phospho_WB_112922
    Whole cell extracts (15 μg protein) from serum-starved Jurkat cells untreated (-) or treated (+) with 100 ng/mL PMA for 5 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone Poly21A07) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG (Cat. No. 410406) at a 1:3000 dilution. Equal ERK1/2 loading was confirmed by probing membranes with purified anti-ERK1/2 (clone W15133A) (Cat. No. 687002) at 1.0 µg/mL. Lane M: Molecular weight marker
  • B_Poly21A07_PURE_ERK1-2-Phospho_WB_2_112922
    Whole cell extracts (15 μg protein) from serum-starved Raw264.7 cells untreated (-) or treated (+) with 100 ng/mL PMA for 1 hour were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone Poly21A07) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG (Cat. No. 410406) at a 1:3000 dilution. Equal ERK1/2 loading was confirmed by probing membranes with purified anti-ERK1/2 (clone W15133A) (Cat. No. 687002) at 1.0 µg/mL. Lane M: Molecular weight marker
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604951 50 µL £121
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604952 200 µL £241
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Description

ERK1/2 (extracellular signal-regulated protein kinase) are members of the mitogen-activated protein kinase (MAPK) family of serine/threonine kinases. ERK1, also called Mitogen-activated protein kinase 3 (MAPK3) and ERK2, also called Mitogen-activated protein kinase 1 (MAPK1), are both integral effectors of the Ras-dependent extracellular signal-regulated kinase 1 (ERK1/2) mitogen-activated (MAP) kinase pathway. As such, they are vital regulators of cell cycle, differentiation, proliferation, stress-response, survival, and senescence. ERK1/2 are activated via phosphorylation of their tyrosine (Tyr204) and threonine (Thr202) residues by MEK1 and MEK2 in response to mitogenic stimuli. Upon activation, ERK1/2 translocate to the nucleus where they function in the regulation of transcription and translation. Dysregulation of ERK1/2 and the hyper-activation of the ERK pathway are characteristics of many cancers where it contributes to tumorigenesis, metastasis, and treatment-resistance, making ERK1/2 an important therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide of human Erk1/2 phosphorylated at Thr202/Tyr204
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Use 20 μL per 10 mL antibody dilution buffer for each gel.

Application Notes

This clone was tested by ICC in HeLa cells treated with or without 100 ng/mL PMA. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. All three methods resulted in weak staining and a lack of phospho-specificity.

RRID
AB_2927813 (BioLegend Cat. No. 604951)
AB_2927813 (BioLegend Cat. No. 604952)

Antigen Details

Structure
ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD. They share 85% homology.
Distribution

Ubiquitously expressed/ Localized to the cell membrane and nucleus

Function
Serine/threonine-protein kinase, phosphatase
Interaction
Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Busca R, et al. 2016. Front. Cell Dev. Biol. 4:53.
  2. Meloche S and Pouyssegur J. 2007. Oncogene. 26:3227.
  3. Wortzel I and Seger R. 2011. Genes and Cancer. 3:195.
Gene ID
5594 View all products for this Gene ID 5595 View all products for this Gene ID
UniProt
View information about ERK1/2 on UniProt.org
Go To Top Version: 1    Revision Date: 08/31/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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