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Purified anti-ERK1 Antibody

Pricing & Availability
Clone
W15133A (See other available formats)
Regulatory Status
RUO
Other Names
Extracellular signal-regulated kinase 1, Mitogen-activated protein kinase 3 (MAPK3), p44ERK1, p44-MAPK, PRKM3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W15133A_PURE_ERK1_Antibody_WB_042516_updated
Total lysates (15µg protein) from 293T, 293T/ERK1 knockout (KO) and 293T/ERK2 knockdown (KD) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-ERK1 antibody, clone W15133A (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-ERK1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 1_W15133A_PURE_ERK1_Antibody_WB_042516_updated
    Total lysates (15µg protein) from 293T, 293T/ERK1 knockout (KO) and 293T/ERK2 knockdown (KD) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-ERK1 antibody, clone W15133A (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-ERK1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 2_W15133A_PURE_ERK1_Antibody_WB_042516
    Total cell lysate from human Raji cells (lane 1), Jurkat cells (lane 2), and mouse A20 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ERK1 (clone W15133A) antibody. Proteins were visualized using an HRP Goat anti-rat IgG antibody and chemiluminescence detection.
  • 3_W15133A_PURE_ERK1_IHC-P_Antibody_072122
    IHC staining using purified anti-Erk1 antibody (clone W15133A) on formalin-fixed paraffin-embedded human colon carcinoma tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG antibody (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 µM.
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687002 100 µg £182
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Description

ERK1 is a member of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1 and ERK2 share 85% homology and both are involved in the MAP kinase signaling cascade. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Product was tested in IHC using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20

RRID
AB_2616892 (BioLegend Cat. No. 687002)

Antigen Details

Structure
379 amino acids with a predicted molecular weight of 43.1 kD. Contains a kinase domain responsible for phosphorylation of down stream substrates.
Distribution

Nucleus and cytoplasm.

Function
ERK1 is a serine/threonine kinase which acts as a key component of the MAP kinase signaling pathway. ERK1 and ERK2 have been shown to play redundent roles in cell proliferation, differentiation, migration, survival, and apoptosis.
Interaction
Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5595 View all products for this Gene ID
UniProt
View information about ERK1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All ERK1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-ERK1 W15133A WB,KO/KD-WB,IHC-P
Go To Top Version: 2    Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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