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Purified anti-ERK2 Antibody

Pricing & Availability
Clone
Poly6242 (See other available formats)
Regulatory Status
RUO
Other Names
Mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, p41mapk, p42 MAP Kinase
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
a-poly6242_purified_ERKAntibody_1_032019
Whole cell extracts (15 µg protein) from RPMI 8226 (reduced expression control), HeLa, K562, and NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with a 1:2000 dilution of Purified anti-ERK2 Antibody, clone Poly6242, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Predicted MAPK1 expression obtained from Human Protein Atlas. Lane M: Molecular Weight marker.
  • a-poly6242_purified_ERKAntibody_1_032019
    Whole cell extracts (15 µg protein) from RPMI 8226 (reduced expression control), HeLa, K562, and NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with a 1:2000 dilution of Purified anti-ERK2 Antibody, clone Poly6242, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Predicted MAPK1 expression obtained from Human Protein Atlas. Lane M: Molecular Weight marker.
  • b-Poly6242_IHC_062508
    Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6242 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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624201 50 µL £81
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Description

ERK2 is a 42 kD dual specificity kinase also known as mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, and p41mapk. The MAPK family includes several subgroups encompassing the ERK, JNK, and p38 kinases. ERK is phosphorylated in response to various extracellular signals inducing homodimerization and nuclear translocation where it phosphorylates target proteins regulating cell growth and survival. ERK2 interacts with a number of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others. The Poly6242 antibody recognizes human, mouse, and rat ERK2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide mapping to the carboxy terminus of rat ERK2
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 50% glycerol, and 0.1% gelatin.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IHC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml of antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Additional Product Notes

The 50 µL size can be used for approximately 5 Western blots.

RRID
SCR_001134 (BioLegend Cat. No. 624201)

Antigen Details

Structure
Mitogen-activated protein kinase family member, dual specificity kinase, homodimer; approximately 42 kD
Distribution

Ubiquitous cytoplasmic protein, translocates to nucleus upon activation.

Function
Dual specificity kinase involved in eukaryotic signal transduction. Phosphorylated ERK2 homodimerizes and translocates from the cytoplasm to the nucelus. Activated ERK2 phosphorylates various targets to regulate cell proliferation, survival and apoptosis.
Interaction
Interacts with a variety of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others.
Modification
Auto-phosphorylation on multiple sites (threonine, serine, tyrosine)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Khokhlatchev AV, et al. 1998. Cell 93:605.
2. Boulton TG, et al. 1991. Cell 65:663.

Regulation
Extracellular signals including cytokine receptor engagement, growth factor engagement, and neuropeptides induce phosphorylation and activation of ERK2.
Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK2 on UniProt.org
Go To Top Version: 3    Revision Date: 03/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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