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Purified anti-ERp57 Antibody

Pricing & Availability
Clone
W19080B (See other available formats)
Regulatory Status
RUO
Other Names
Protein Disulfide Isomerase Family A Member 3, PDIA3, Endoplasmic Reticulum Resident Protein 57, GRP58, ER Protein 57, GRP57, Epididymis Secretory Protein Li 269, Epididymis Scretory Sperm Binding Protein Li 93n
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19080B_PURE_ERp57_Antibody_1_061020
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERp57 antibody (clone W19080B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19080B_PURE_ERp57_Antibody_1_061020
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERp57 antibody (clone W19080B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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937301 25 µg £70
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937302 100 µg £174
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Description

ERp57 is a member of the protein disulfide family of endoplasmic reticulum localized oxidoreductases and plays an important role in disulfide bond formation and protein folding. Along with calnexin and calreticulin, it is essential for the synthesis and quality control of nascent glycoproteins in the secretory pathway. It is also a core component of the peptide-loading complex that is required for MHC class I presentation. ERp57 is upregulated in multiple neurodegenerative diseases and cancers, and targeted disruption of ERp57 sensitizes cancer cells to chemotherapeutic agents and ionizing radiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human ERp57 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed IP testing.

Product Citations
  1. Khaleafi R, et al. 2023. Front Immunol. 1231782:14. PubMed
RRID
AB_2861126 (BioLegend Cat. No. 937301)
AB_2861126 (BioLegend Cat. No. 937302)

Antigen Details

Structure
ERp57 is a 505 amino acid protein with a predicted molecular weight of 57 kD.
Distribution

Endoplasmic reticulum/Ubiquitously expressed

Function
Molecular chaperone
Biology Area
Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Endoplasmic Reticulum Markers, Enzymes and Regulators
Antigen References
  1. Oliver JD, et al. 1997. Science. 275:86-8.
  2. Kienast A, et al. 2007. Nat Immunol. 8:864-72.
  3. Hussman M, et al. 2015. Oncotarget. 6:39247-61.
  4. Blees A, et al. 2017. Nature. 551:525-528.
Gene ID
2923 View all products for this Gene ID
UniProt
View information about ERp57 on UniProt.org
Go To Top Version: 1    Revision Date: 06/10/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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