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Purified anti-ETS1 Antibody

Pricing & Availability
Clone
W17042D (See other available formats)
Regulatory Status
RUO
Other Names
ETS-1, ETS proto-oncogene 1, Protein C-ets-1, EWSR2, FLJ10768, p54, ETS Proto-Oncogene 1, V-Ets Avian Erythroblastosis Virus E26 Oncogene Homolog 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17042D_PURE_ETS1_Antibody_080519
Whole cell extracts (15 µg protein) from the indicated cell lines, BeWo (low expressor control), HaCaT, PC-3, MOLT-4, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ETS1 antibody, clone W17042D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted ETS1 expression was obtained from Human Protein Atlas.
  • W17042D_PURE_ETS1_Antibody_080519
    Whole cell extracts (15 µg protein) from the indicated cell lines, BeWo (low expressor control), HaCaT, PC-3, MOLT-4, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ETS1 antibody, clone W17042D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted ETS1 expression was obtained from Human Protein Atlas.
Cat # Size Price Quantity Check Availability Save
667901 25 µg £70
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667902 100 µg £174
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Description

ETS1 is a transcription factor that regulates gene transcription of cytokine and chemokine genes. The expression of ETS1 is modulated by transcription factors and kinases. It is expressed in lymphoid organs where it may  play a role in cell differentiation, survival, and proliferation. The ETS1 gene encodes multiple proteins by  alternative splicing of mRNA, resulting in at least six distinctive isoforms; the major species are p51, p48, p42, and p39. In certain cancers, elevated ETS1 expression is a predictive marker of poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic human ETS1 peptide surrounding amino acid 170.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W17042D failed ICC testing; PC3 cells were fixed with 4% paraformaldehyde and tested using two  permeabilization methods (Methanol and Trition X-100). The clone failed ICC testing due to weak and non-specific staining of cytosol.

 

IP has not been tested on clone W17042D.

RRID
AB_2814508 (BioLegend Cat. No. 667901)
AB_2814508 (BioLegend Cat. No. 667902)

Antigen Details

Structure
ETS1 is a 441 amino acid protein with a predicted molecular weight of 51 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Transcription factor that regulates cytoskine and chemokine genes.
Biology Area
Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Bories JC, et al. 1995. Nature. 377:635-8. 
  2. Ho IC, et al. 1990. Science. 250:814-8. 
Gene ID
2113 View all products for this Gene ID
UniProt
View information about ETS1 on UniProt.org
Go To Top Version: 1    Revision Date: 08/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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