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Purified anti-ETS2 Antibody

Pricing & Availability
Clone
W15163A (See other available formats)
Regulatory Status
RUO
Other Names
v-ets avian erythroblastosis virus E26 oncogene homolog 2, Protein C-ets-2, ETS proto-oncogene 2, ETS2IT1
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15163A_Purified_ETS2_Antibody_WB_113016
Total cell lysate from human MCF7 cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of purified anti-ETS2 antibody (clone W15163A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. MW*: protein standards with longer exposure time.
  • W15163A_Purified_ETS2_Antibody_WB_113016
    Total cell lysate from human MCF7 cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of purified anti-ETS2 antibody (clone W15163A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. MW*: protein standards with longer exposure time.
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693202 100 µg £174
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Description

ETS erythroblastosis virus E26 oncogene homologue 2 (ETS2), also known as ETS2IT1, is a member of the ETS transcription factor family. ETS2 contains one conserved winged helix-turn-helix ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T. ETS2 has been found to be an evolutionary conserved proto-oncogene and is a downstream regulator of the Ras/Raf/MAPK pathway. ETS2 participates in the regulation of the genes involved in stem cell development, cell senescence, cell death, apoptosis, and tumorigenesis. Defects in ETS2 gene have been associated with choriocarcinoma and Down syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human ETS2 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632833 (BioLegend Cat. No. 693202)

Antigen Details

Structure
469 amino acids with a predicted molecular weight of 53 kD. Contains an ETS DNA-binding domain and a PNT (pointed) domain.
Distribution
Nucleus.
Function
ETS2 is a transcription factor downstream of the Ras/Raf/MAPK pathway. ETS2 is a proto-oncogene involved in stem cell development, cell senescence, cell death, apoptosis, and tumorigenesis.
Interaction
Interacts with CDK10, RFWD2, TWIST1, and TWIST2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Transcription Factors
Antigen References

1. Carrero ZI, et al. 2016. Oncotarget. 7:12554.
2. Donnison M, et al. 2015. Dev. Biol. 397:77.
3. Guen VJ, et al. 2013. Proc. Natl. Acad. Sci. USA 110:19525.
4. Polydorou C, Georgiades P. 2013. Nat. Commun. 4:1658.
5. Do PM, et al. 2012. Genes Dev. 26:830.
6. Zabuawala T, et al. 2010. Cancer Res. 70:1323.

Gene ID
2114 View all products for this Gene ID
UniProt
View information about ETS2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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