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Purified anti-FABP1 Antibody

Pricing & Availability
Clone
Poly28601 (See other available formats)
Regulatory Status
RUO
Other Names
FABP1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28601_Purified_FABP1_Antibody_1_052119
Western blot of purified anti-FABP1 antibody (Poly28601). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 40 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28601_Purified_FABP1_Antibody_1_052119
    Western blot of purified anti-FABP1 antibody (Poly28601). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 40 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28601_Purified_FABP1_Antibody_2_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28601_Purified_FABP1_Antibody_3_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28601_Purified_FABP1_Antibody_4_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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860101 25 µg £86
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Description

Fatty acid binding protein 1 (FABP1) is involved in fatty acid binding, uptake, transport, and metabolism. FABP1 binds to cholesterol, free fatty acids, and bilirubin. Diseases associated with FABP1 include Hepatocellular Adenoma, Fatty Liver Disease and Nonalcoholic 1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Recombinant Fatty Acid Binding Protein 1 (FABP1) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810737 (BioLegend Cat. No. 860101)

Antigen Details

Structure
FABP1 is a 127 amino acid protein with an apparent molecular mass of ~ 14 kD.
Distribution

Tissue Distribution: Liver, kidney, colon, rectum
Cellular Distribution: Cytosol and nucleus

Function
FAPB1 is involved in fatty acid uptake, transport, and metabolism.
Interaction
Cholesterol, free fatty acids, bilirubin
Cell Type
Mesenchymal cells
Biology Area
Cell Biology, Cell Proliferation and Viability
Molecular Family
Carrier Proteins
Antigen References
  1. Amiri M, et al. 2018. Gene. 676:171.
  2. Xu Y, et al. 2015. Clin Chim Acta. 445:85.
  3. Thumser AE, et al. 2014. Curr Opin Clin Nutr Metab Care. 17(2):124.
Gene ID
32168 View all products for this Gene ID 14080 View all products for this Gene ID 24360 View all products for this Gene ID
UniProt
View information about FABP1 on UniProt.org
Go To Top Version: 2    Revision Date: 06/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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