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Purified anti-FBXO7 Antibody

Pricing & Availability
Clone
W16207A (See other available formats)
Regulatory Status
RUO
Other Names
F-box only protein 7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16207A_PURE_FBXO7_Antibody_1_052118
Western blot of purified anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 3 ng of GST-tagged human recombinant FBXO7; Lane 3: 20 µg of Hela cell lysate; Lane 4: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W16207A_PURE_FBXO7_Antibody_1_052118
    Western blot of purified anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 3 ng of GST-tagged human recombinant FBXO7; Lane 3: 20 µg of Hela cell lysate; Lane 4: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W16207A_PURE_FBXO7_Antibody_2_052118
    ICC staining of purified anti-FBXO7 antibody (clone A16207A) on SH-SY5Y cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then co-stained with 5 µg/ml of the anti-FBXO7 and anti-α-tubulin (Cat. No. 627902) antibodies overnight at 4°C, followed by incubation wit 5 µg/ml of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) and Alexa Fluor® 488 goat anti-mouse IgG (Cat. No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured with a 60X objective. Scale bar: 20 µm
  • W16207A_PURE_FBXO7_Antibody_3_052118
    Western blot of purified anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate; Lane 4: 20 µg of NIH3T3 cell lysate; Lane 5: 20 µg of PC12 cell lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Cytochrome C (Cat. No. 612504) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • W16207A_PURE_FBXO7_Antibody_4_052118
    Western blot of purified anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Cytochrome C (Cat. No. 612504) was used as loading control. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
853501 25 µg £77
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853502 100 µg £197
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Description

F-box only protein 7 (FBXO7) is involved in the ubiquitination process during protein degradation. FBXO7 is a subunit of the SCF (SKP1/cullin‐1/F‐box protein) complex. The scaffold proteins cullin‐1 and SKP1, the RING subunit Rbx1 together with the substrate‐recruiting subunit FBXO7 form a functional E3 ubiquitin ligase. FBXO7 is implicated in Parkinson’s disease. In addition to reduced proteasome activity, loss of FBXO7 in mice leads to Parkinsonism-like phenotype. Human FBXO7 is mainly localized to the nucleus, while the mutation observed in patients with parkinsonian-pyramidal syndrome leads to impaired nuclear localization of this protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human FBXO7 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for human FBXO7 and does not cross-react with rat or mouse FBXO7, verified by western blot. The two bands observed in western blot potentially indicate the two isoforms of FBXO7.

RRID
AB_2734650 (BioLegend Cat. No. 853501)
AB_2734650 (BioLegend Cat. No. 853502)

Antigen Details

Structure
FBXO7 is a 522 amino acid protein with a molecular mass of ~60 kD.
Distribution

Tissue distribution: FBXO7 is ubiquitously expressed in the many tissues including brain and muscle.
Cellular distribution: Cytoplasmic and nucleus.

Function
FBXO7 is involved in the ubiquitin-proteasome system, and defects in FBXO7 cause Parkinson's disease-like phenotypes.
Interaction
FBXO7 interacts with the members of the SCF (SKP1/cullin‐1/F‐box protein) complex.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Nuclear Markers
Antigen References
  1. Vingill S, et al. 2016. EMBO J. 35: 2008-25.
  2. Zhao T, et al. 2011. PLoS One. 6:e16983.
  3. Zhou ZD, et al. 2015. Hum. Mol. Genet. 24:6314-30.
Gene ID
25793 View all products for this Gene ID
UniProt
View information about FBXO7 on UniProt.org
Go To Top Version: 1    Revision Date: 05/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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