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Purified anti-Fibrinogen Antibody

Pricing & Availability
Clone
G8 1-2 (See other available formats)
Regulatory Status
RUO
Other Names
Fibrinogen Alpha Chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
G81-2_PURE_Fibrinogen_Antibody_1_102220
Western blot of purified anti-Fibrinogen antibody (clone G8 1-2). Lane 1, 5: Molecular weight marker; Lane 2: 2 µg of human liver lysate; Lane 3: 20 µg of HepG2 lysate; Lane 4: 20 µg of MCF7 cell lysate. The blot was incubated with 0.2 µg/mL primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending EGFR expression; predicted expression data was obtained from Human Protein Atlas.
  • G81-2_PURE_Fibrinogen_Antibody_1_102220
    Western blot of purified anti-Fibrinogen antibody (clone G8 1-2). Lane 1, 5: Molecular weight marker; Lane 2: 2 µg of human liver lysate; Lane 3: 20 µg of HepG2 lysate; Lane 4: 20 µg of MCF7 cell lysate. The blot was incubated with 0.2 µg/mL primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending EGFR expression; predicted expression data was obtained from Human Protein Atlas.
  • G81-2_PURE_Fibrinogen_Antibody_2_102220
    ICC staining of purified anti-Fibrinogen antibody (clone G8 1-2) on HepG2 cell. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/mL primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm.
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871801 25 µg £73
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871802 100 µg £189
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Description

Fibrinogen is a glycoprotein that is synthesized by hepatocytes in the liver. Fibrinogen alpha chain (FGA) is the alpha component of the fibrinogen, which is released to plasma after fibrinogen is cleaved by thrombin. Upon vascular injury, FGA forms the fibrin complex with fibrinogen beta (FGB) and fibrinogen gamma (FGG). The complex is one of the primary components of blood clots. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL.  For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for fibrinogen alpha chain.

RRID
AB_2888778 (BioLegend Cat. No. 871801)
AB_2888778 (BioLegend Cat. No. 871802)

Antigen Details

Structure
FGA is a 866 amino acid protein with predicted molecular mass of ~84 kD and observed molecular mass of ~62 kD.
Distribution

Tissue distribution: Liver, plasma
Cellular distribution: Extracellular, plasma membrane, endoplasmic reticulum

Function
FGA form fibrin complex with fibrinogen beta (FGB) and fibrinogen gamma (FGG). This complex is the primary components for blood clots.
Interaction
Forms homohexamer with 2-3 non-identical chains. Interacts with PLAT
Cell Type
Plasma cells, Platelets
Biology Area
Cell Biology, Immunology, Neuroinflammation
Molecular Family
Adhesion Molecules
Antigen References
1. Yazaki M, et al. 2018. Int. J. Mol. Sci. 19(1)
2. Lebrton A, et al. 2016. Ann. Biol. Clin., Paris. 74(4):405
3. Fish RJ, et al. 2012. Thromb. Haemost. 108(3):419
Gene ID
2243 View all products for this Gene ID
UniProt
View information about Fibrinogen on UniProt.org
Go To Top Version: 1    Revision Date: 10/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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