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Purified anti-Flotillin-1 Antibody

Pricing & Availability
Clone
W16108A (See other available formats)
Regulatory Status
RUO
Other Names
Flotillin 1, Flot-1, Integral Membrane Component of Caveolae
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16108A_PURE_Flotillin-1_Antibody_WB_101317
Western blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20µg; Myc-tagged Flotillin-1 or GST-tagged Flotillin-2 recombinant protein: 20 ng.
  • 1_W16108A_PURE_Flotillin-1_Antibody_WB_101317
    Western blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20µg; Myc-tagged Flotillin-1 or GST-tagged Flotillin-2 recombinant protein: 20 ng.
  • 2_W16108A_PURE_Flotillin-1_Antibody_IF_101317
    ICC staining of anti-Flotillin-1 antibody (clone W16108A) on Hela cells. The cells were transfected with plasmids carrying CRISPR gRNA against Flotillin-1, Cas9 nuclease, and GFP. Two days after transfection the cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI. Arrowheads: transfected cells. Images were captured with a 60X objective. Scale bar: 25 µm.
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849801 25 µg £89
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849802 100 µg £221
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Description

The flotillin family is composed of two highly homologous proteins, flotillin-1 and flotillin-2 that share ~50% amino acid sequence similarity. Flotillins are ubiquitously expressed, membrane-associated proteins, and were shown to be associated with lipid microdomains called membrane rafts. Flotillins are present in various cellular compartments, including the plasma membrane and endosomes, and they have been suggested to function in cellular signaling and membrane trafficking processes, including endocytosis and endosomal trafficking.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry microscopy, a concentration range of 1.0-5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody specifically recognizes Flotillin-1 but not Flotillin-2, verified using WB.

Product Citations
  1. Buenaventura T, et al. 2019. PLoS Biol. 17:e3000097. PubMed
RRID
AB_2687309 (BioLegend Cat. No. 849801)
AB_2687309 (BioLegend Cat. No. 849802)

Antigen Details

Structure
Flotillin-1 is a 427 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Flotillin-1 is ubiquitously expressed. </br>Cellular distribution: Flotillin-1 is mainly expressed in the cytosol and endosomes.

Function
Flotillins are thought to interact with the cytoskeleton and function in a number of cellular processes such as endocytosis.
Interaction
Flotillin-1 interacts with cbl-associated protein/ponsin (CAP).
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Antigen References

1. Otto G et al., 2011. Journal of Cell Science. 124: 3933.

Gene ID
10211 View all products for this Gene ID
UniProt
View information about Flotillin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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