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Purified anti-FMRP Antibody (Previously Covance catalog# MMS-5232)

Pricing & Availability
Clone
5C2 (See other available formats)
Regulatory Status
RUO
Other Names
Fragile X mental retardation protein 1, POF, FMRP, POF1, FRAXA
Previously
Covance Catalog# MMS-5232
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_5C2_Pure_FMRP_Antibody_WB_022521
Human brain or whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:1000 dilution) purified anti-FMRP antibody (clone 5C2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 1_5C2_Pure_FMRP_Antibody_WB_022521
    Human brain or whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:1000 dilution) purified anti-FMRP antibody (clone 5C2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 2_5C2_Pure_FMRP_Antibody_1_09518
    Western blot of purified anti-FMRP antibody (clone 5C2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3_5C2_Pure_FMRP_Antibody_2_09518
    ICC staining of purified anti-FMRP antibody (clone 5C2) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation wit 5 µg/ml of Alexa Fluor® 488 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_5C2_Pure_FMRP_Antibody_3_09518
    IHC staining of purified anti-FMRP antibody (clone 5C2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hours at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5_5C2_Pure_FMRP_Antibody_4_09518
    IHC staining of purified anti-FMRP antibody (clone 5C2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hours at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 6_5C3_Purified_FMRP_WB_2_021315
    Reactivity of 5C2 to cell lysates of lymphoblastoid cell lines derived from normal (C) and male FX full mutation (FM) individuals @ 1:2000 dilution. The absence of a band in the FX full mutation lane shows the protein is not present in fragile X individuals but is present in normal subjects. (Courtesy of R. Kascsak)
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834702 25 µL £73
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834701 100 µL £189
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Description

The gene encoding Fragile X mental retardation protein 1 (FMRP or FMR1), also known as premature ovarian failure 1 (POF1), contains multiple CGG sequence repeats that are positively correlated with impaired cognitive and reproductive function. FMRP may be involved in mRNA transport and synaptic protein synthesis. The gene that encodes FMRP is located in the X chromosome, and an abnormally large number of CGG repeats (200 - 1000 times) causes Fragile X syndrome due to inadequate amounts of FMRP, leading to severe learning deficiency and mental retardation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a recombinant protein isolated from baculovirus expressing mouse FMRP.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P) and immunocytochemistry (ICC).

The epitope for 5C2 lies between amino acids 347-353 of FMRP. This clone does not cross react with FXR1 or FXR2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gholizadeh S, et al. 2014. Neuropsychopharmacology. 39:3100.
Product Citations
  1. Kalinowska M, et al. 2022. Mol Autism. 13:29. PubMed
  2. Raj N, et al. 2021. Cell Reports. 35(2):108991. PubMed
  3. Graef JD, et al. 2020. Eur J Neurosci. 51:2143. PubMed
  4. Liu XS, et al. 2018. Cell. 172:979. PubMed
RRID
AB_2564993 (BioLegend Cat. No. 834702)
AB_2564993 (BioLegend Cat. No. 834701)

Antigen Details

Structure
Expected MW: 68-80 kD (smaller truncations of FMRP seen at lower molecular weights)
Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Gene ID
2332 View all products for this Gene ID
UniProt
View information about FMRP on UniProt.org

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Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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