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Purified anti-FOXA2 Antibody

Pricing & Availability
Clone
P87H4B7 (See other available formats)
Regulatory Status
RUO
Other Names
Hepatocyte nuclear factor 3-beta, HNF-3-beta, TCF-3B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P87H4B7_PURE_FOXA2_Antibody_1_WB_041916
Total cell lysate from HepG2 (lane 1, 15 µg) and HeLa (lane 2, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-FOXA2 (clone P87H4B7) antibody. Proteins were visualized using an HRP goat anti-mouse IgG (minimal x-reactivity) antibody and chemiluminescence detection.
  • P87H4B7_PURE_FOXA2_Antibody_1_WB_041916
    Total cell lysate from HepG2 (lane 1, 15 µg) and HeLa (lane 2, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-FOXA2 (clone P87H4B7) antibody. Proteins were visualized using an HRP goat anti-mouse IgG (minimal x-reactivity) antibody and chemiluminescence detection.
  • P87H4B7_PURE_FOXA2_Antibody_2_ICC_050319
    A549 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for 5 minutes followed by blocking with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL anti-FOXA2 (clone P87H4B7) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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685802 100 µg £197
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Description

FOXA2 belongs to the Forkhead box family. This transcription factor is also known as Hepatocyte Nuclear Factor 3 beta (HNF-3β). It is involved in mouse embryonic development, differentiation and maturation of endoderm derived organs, and glucose and lipid metabolism. Loss of FOXA2 has been connected to diseases such as hyperinsulinemic hypoglycemia and lung adenocarcinoma metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human FOXA2 expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. For immunocytochemistry, a concentration range of 4.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chen YF, et al. 2020. Sci Adv. 6:eaay0264. PubMed
  2. Truong DJ, et al. 2021. Nat Cell Biol. 23:652. PubMed
RRID
AB_2616799 (BioLegend Cat. No. 685802)

Antigen Details

Structure
457 amino acids with a predicted molecular weight of approximately 48 kD. An isoform was identified with the predicted molecular weight of approximately 49 kD.
Distribution

Nucleus (but not nucleoli).

Function
Interact with the cis-acting regulatory regions of several liver-specific genes. FOXA2 is important for notochord development in embryo. It is also involved in regulation of fat metabolism, glucose homeostasis, TH2-mediated pulmonary inflammation and goblet cell hyperplasia.
Interaction
DNA and chromatin.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Transcription Factors
Antigen References

1. Arenas E. 2008. Cell Stem Cell. 2:110.
2. Sun L, et al. 2015. World J. Methodol. 5:223.
3. Li CM, et al. 2015. Genes Dev. 29:1850.
4. Sund NJ, et al. 2001. Genes Dev. 15:1706.
5. Levinson-Dushnik M, Benvenisty N. 1997. Mol. Cell Biol. 17:3817.

Gene ID
3170 View all products for this Gene ID
UniProt
View information about FOXA2 on UniProt.org
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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