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Purified anti-FOXP1 (Forkhead Box P1) Antibody

Pricing & Availability
Clone
O94D2 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead Box P1, Fork Head-Related Protein Like B, QRF1, hFKH1B, MFH, 12CC4, HSPC215
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O94D2_purified_FOXP1_Antibody_1_112818
Total cell lysates (15 µg total protein) from PC-3, HepG2, SH-SY5Y (reduced expression control), and HaCat (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-FOXP1 Antibody, clone O94D2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • O94D2_purified_FOXP1_Antibody_1_112818
    Total cell lysates (15 µg total protein) from PC-3, HepG2, SH-SY5Y (reduced expression control), and HaCat (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-FOXP1 Antibody, clone O94D2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
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679001 25 µg £77
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679002 100 µg £197
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Description

FOXP1 (Forkhead Box P1) is a transcription regulator, which plays an important role in the brain, cardiac, lung, and lymphocyte development. Mutations in FOXP1, which are located at 3p13, have been reported in patients with global developmental delay (GDD), intellectual disability (ID), and speech defects. FOXP1 is targeted by recurrent chromosome translocations and its overexpression confers a poor prognosis in a number of types of lymphomas, suggesting it may function as an oncogene. In contrast, FOXP1 localizes to a tumor suppressor locus at 3p14.1 and the loss of FOXP1 expression in breast cancer is associated with a worse outcome, suggesting FOXP1 may function as a tumor suppressor in other tissue types. Recent studies also suggest that in embryonic stem cells (ESC), specific isoforms of FOXP1 help maintain the ESC pluripotency and stimulates the expression of transcription factor genes that are required for pluripotency such as OCT4, NANOG, NR5A2, and GDF3, while concomitantly repressing genes required for ESC differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human FOXP1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2566074 (BioLegend Cat. No. 679001)
AB_2566074 (BioLegend Cat. No. 679002)

Antigen Details

Structure
Different isoforms with a predicted molecular weight of approximately 55 to 85 kD with the characteristics of forkhead DNA-binding domains.
Distribution

Brain, heart, lung, lymphocyte, and the nucleus.

Function
Transcription regulator that plays an important role in cardiac, lung, and lymphocyte development.
Interaction
CTBP1, FOXP2, and FOXP4.
Cell Type
B cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Antigen References

1. Le Fevre AK, et al. 2013. Am. J. Med. Genet. A. 161A:3166.
2. Ijichi N, et al. 2013. Vitam. Horm. 93:203.
3. Koon HB, et al. 2007. Expert Opin. Ther. Targets 11:955.
4. Gabut M, et al. 2011. Cell 1:132.
5. Mraz M, et al. 2014. Blood 124:84.

Gene ID
27086 View all products for this Gene ID
UniProt
View information about FOXP1 on UniProt.org
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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