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Purified anti-FUS Antibody

Pricing & Availability
Clone
10F7 (See other available formats)
Regulatory Status
RUO
Other Names
FUS, TLS
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10F7_PURE_FUS_Antibody_090418
Western blot of purified anti-FUS antibody (clone 10F7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 10F7_PURE_FUS_Antibody_090418
    Western blot of purified anti-FUS antibody (clone 10F7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 10F7_PURE_FUS_Antibody_2_122123
    IHC staining of Purified anti-FUS (clone 10F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Purified anti-FUS (clone 10F7) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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855001 25 µg £73
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855002 100 µg £189
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Description

FUS/TLS is an RNA-binding protein that was originally discovered as a fusion protein caused by chromosomal translocation in cancer. FUS/TLS is as an important member of the cellular DNA repair response. The C-terminal end of FUS is involved in protein and RNA binding, while the N-terminal motif of FUS plays a role in transcriptional activation. FUS was also shown to accumulate in the cytoplasm of neurons under hyperosmotic conditions, and FUS mislocalization has been implicated as being the first step toward disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLS/FUS recombinant protein fused with GST tag.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2750473 (BioLegend Cat. No. 855001)
AB_2750473 (BioLegend Cat. No. 855002)

Antigen Details

Structure
FUS is a 526 amino acid protein with an apparent molecular mass of ~ 53 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and cytosol

Function
FUS/TLS binds to DNA and maintains genomic integrity.
Interaction
Interacts with HDAC1, RNA Polymerase II, FUSIP1, ILF3, PRMT1, RELA, SPI1, and TNPO1.
Cell Type
Astrocytes, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Synaptic Biology
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Efimova AD, et al. 2017. Mol Biol (Mosk) 51:387.
  2. Ederle H, Dormann D. 2017. FEBS Lett. 591:1489.
  3. Guerrero EN, et al. 2016. Prog. Neurobiol. 145-146:78.
Gene ID
2521 View all products for this Gene ID 233908 View all products for this Gene ID 217385 View all products for this Gene ID
UniProt
View information about FUS on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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